Rabbit Polyclonal Antibody
Catalog No. P80-65R
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
P80-65R-100 | 100 ug | $270 | |
P80-65R-BULK | BULK | Contact Us |
Overview:
EIF2AK2 (also known as double-stranded RNA-activated protein kinase and PKR) is a protein kinase that is involved in HIV/gp120-associated neurodegeneration (1). EIF2AK2 is a mediator of gp120 neurotoxicity and is a substrate for a family of protein kinases involved in environmental stress responses. Activation of EIF2AK2 by dsRNA leads to autophosphorylation at Thr446/451. Activated EIF2AK2 phosphorylates the alpha subunit of EIF2, which in turn inhibits protein synthesis. EIF2AK2 plays a critical role in mRNA translation, cell proliferation and apoptosis. Crosstalk between EIF2AKs and p53 has implications in cell proliferation and tumorigenesis (2).
References:
1. Baltzis, D. et al: The eIF2alpha kinases PERK and PKR activate glycogen synthase kinase 3 to promote the proteasomal degradation of p53. J. Biol Chem. 2007; 282(43):31675-87
2. Alirezaei, M. et al: Human immunodeficiency virus-1/surface glycoprotein 120 induces apoptosis through RNA-activated protein kinase signaling in neurons. J. Neurosci. 2007;27(41):11047-55.
Specificity:
Recognizes the EIF2AK2 protein phosphorylated at threonine 446
Cross Reactivity:
Human and Mouse
Host / Isotype / Clone#:
Rabbit, IgG
Immunogen:
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr446
Purification:
Affinity chromatography
Stability:
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data:
Western blot analysis of extracts from K562 cells using EIF2AK2 antibody (lanes 1 and 2) and anti-phospho-EIF2AK2 (Thr446) antibody (lanes 3 and 4).
Angiogenesis, Cellular Stress, Inflammation, Neurobiology, Ser/Thr Kinases
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