Specificity:

Recognizes the PLCG2 protein phosphorylated at tyrosine 753

Cross Reactivity:

Human, Mouse and Rat

Host / Isotype / Clone#:

Rabbit, IgG

Immunogen:

Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr753

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Western blot analysis of extracts from A431 cells untreated or treated with EGF (200ng/ml, 5min) using PLCG2 antibody (lanes 1 and 2) and anti-phospho-PLCG2 (Tyr753) antibody (lanes 3 and 4).

References :

1. Watanabe D. et al: Four tyrosine residues in phospholipase C-gamma 2, identified as Btk-dependent phosphorylation sites, are required for B cell antigen receptor-coupled calcium signaling. J Biol Chem. 2001 Oct 19;276(42):38595-601.

2. Ozdener F. et al: Activation of phospholipase Cgamma2 by tyrosine phosphorylation. Mol Pharmacol. 2002 Sep;62(3):672-9.

3. Kang J S. et al: Cloining and functional analysis of the hematopoetic cell-specific phospholipase C(gamma)2 promoter. FEBS Lett. 1996 Dec 9;399(1-2):14-20.