CHK1 is a 56 kDa serine/threonine protein kinase that was originally identified in fission yeast to play a role in activation of the DNA damage checkpoint in the G2 phase of the cell cycle (1). CHK1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by rad3+, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia (2).
1. Walworth, N. et al: Fission yeast CHK1 protein kinase links the rad checkpoint pathway to cdc2. Nature. 1993 May 27;363(6427):368-71.
2. Walworth, NC. et al: Rad-dependent response of the CHK1-encoded protein kinase at the DNA damage checkpoint. Science. 1996 Jan 19;271(5247):353-6.
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
A Stützer et al., Modulations of DNA Contacts by Linker Histones and Post-translational Modifications Determine the Mobility and Modifiability of Nucleosomal H3 Tails. Molecular Cell January 2016 10.1016/j.molcel.2015.12.015
Liokatis Stamatios et al., Phosphorylation of histone H3 Ser10 establishes a hierarchy for subsequent intramolecular modification events Nature Structural and Molecular Biology July 2012 10.1038/nsmb.2310
Hromas R et al., Chk1 phosphorylation of Metnase enhances DNA repair but inhibits replication fork restart Oncogene September 2012 10.1038/onc.2011.586
Garate Marco et al., Phosphorylation of the tumor suppressor p33ING1b at Ser-126 influences its protein stability and proliferation of melanoma cells FASEB Journal June 2007 10.1096/fj.07-8069com
Cancer, Cell Cycle, Ser/Thr Kinases