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AKT1, Active

Recombinant full-length human AKT1 was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
Catalog No. A16-10G


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Catalog No. Pack Size Price (USD)
A16-10G-05 5 ug $160
A16-10G-10 10 ug $226
A16-10G-BULK BULK Contact Us  


Overview:

AKT1/PKBα is a serine/threonine kinase that belongs to the AKT family. AKT1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). AKT1 is frequently overexpressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas and prostate as well as lymphomas and leukemias (2).


Gene Aliases:

PKB; RAC; PRKBA; MGC99656; RAC-ALPHA


Genbank Number:


References:


1. Coffer, PJ. et al: Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem J. 1998 Oct 1; 335 (Pt 1): 1-13.

2. Anderson, KE. et al: Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91.


Specific Activity:

Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Storage, Stability and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

~85 kDa



Product Datasheets



Safety Datasheet



 Toska E et al., PI3K Inhibition Activates SGK1 via a Feedback Loop to Promote Chromatin-Based Regulation of ER-Dependent Gene Expression Cell Reports April 2019 10.1016/j.celrep.2019.02.111

 Liu X et al., Targeted therapy of the AKT kinase inhibits esophageal squamous cell carcinoma growth in vitro and in vivo International journal of cancer March 2019 10.1002/ijc.32285

 Xie X et al., Gossypetin is a novel MKK3 and MKK6 inhibitor that suppresses esophageal cancer growth in vitro and in vivo Cancer Letters November 2018 30391783

 Patnaik D et al., Structural Basis for Achieving GSK-3β Inhibition with High Potency, Selectivity, and Brain Exposure for Positron Emission Tomography Imaging and Drug Discovery. Journal of Medicinal Chemistry October 2019 10.1021/acs.jmedchem.9b01030

 Ghosh Dastidar Somasish et al., FoxG1 Promotes the Survival of Postmitotic Neurons Journal of Neuroscience January 2011 10.1523/JNEUROSCI.2897-10.2011

 Cohen DE et al., Thioesterase superfamily member 2 promotes hepatic insulin resistance in the setting of glycerol-3-phosphate acyltransferase 1–induced steatosis Journal of Biological Chemistry February 2019 10.1074/jbc.RA118.005184

 Song JH et al., Magnolin targeting of ERK1/2 inhibits cell proliferation and colony growth by induction of cellular senescence in ovarian cancer cells Molecular Carcinogenesis September 2018 10.1002/mc.22909

 Cena Bo et al., The Pim-1 protein kinase is an important regulator of MET receptor tyrosine kinase levels and signaling Molecular and Cellular Biology March 2014 10.1128/MCB.00147-14

 Lamouille Samy et al., TGF-?-induced activation of mTOR complex 2 drives epithelial?mesenchymal transition and cell invasion Journal of Cell Science March 2012 10.1242/jcs.095299

 Li D et al., Resveratrol suppresses colon cancer growth by targeting the AKT/STAT3 signaling pathway Int J Mol Med November 2018 10.3892/ijmm.2018.3969

 Zenga in et al., Lanthionine synthetase C-like protein 2 (LanCL2) is a novel regulator of Akt Molecular Biology of the Cell September 2014 10.1091/mbc.E14-01-0004

 Cennia Vittoria et al., Ankrd2/ARPP is a novel Akt2 specific substrate and regulates myogenic differentiation upon cellular exposure to H2O2 Molecular Biology of the Cell July 2011 10.1091/mbc.E10-11-0928

 Ling Dai Chun et al., Inhibition of Protein Synthesis Alters Protein Degradation through Activation of Protein Kinase B (AKT) Journal of Biological Chemistry July 2013 10.1074/jbc.M112.445148

 Fukuda Koichi et al., Biochemical, Proteomic, Structural, and Thermodynamic Characterizations of Integrin-linked Kinase (ILK) CROSS-VALIDATION OF THE PSEUDOKINASE* Journal of Biological Chemistry June 2011 10.1074/jbc.M111.240093

 Bavelloni Alberto et al., Prohibitin 2 represents a novel nuclear AKT substrate during all-trans retinoic acid-induced differentiation of acute promyelocytic leukemia cells FASEB Journal February 2014 10.1096/fj.13-244368

 Pecorari Luisa et al., Elongation Factor 1 alpha interacts with phospho-Akt in breast cancer cells and regulates their proliferation, survival and motility Molecular Cancer August 2009 10.1186/1476-4598-8-58

 M. Singel Stina et al., KIF14 Promotes AKT Phosphorylation and Contributes to Chemoresistance in Triple-Negative Breast Cancer Neoplasia March 2014 10.1016/j.neo.2014.03.008

 Treebak Jonas et al., Identification of a novel phosphorylation site on TBC1D4 regulated by AMP-activated protein kinase in skeletal muscle American Journal of Physiology - Cell Physiology November 2009 10.1152/ajpcell.00297.2009

 SC Stolze et al., Photo-crosslinking of clinically relevant kinases using H89-derived photo-affinity probes Molecular BioSystems May 2016 10.1039/c6mb00257a

 Asakura Keisuke et al., The Cytostatic Effects of Lovastatin on ACC-MESO-1 Cells Journal of Surgical Research October 2011 10.1016/j.jss.2011.06.037

 Y Zhang et al., Akt mediated phosphorylation of LARP6; critical step in biosynthesis of type I collagen. Science Reports March 2016 10.1038/srep22597


RESEARCH AREAS

AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, WNT Signaling


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