Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
B11-63BR-100	100 ug	$595
B11-63BR-BULK	BULK	Contact Us

Catalog No.

Pack Size

Price (USD)

B11-63BR-100

100 ug

$595

B11-63BR-BULK

BULK

Overview:

BUB1 is a kinase which involved in spindle checkpoint function that in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. BUB1 is highly expreesed in thymus, ovary, and testis (1). BUB1 maintains the steady-state level and centromeric localization of SGO1, and they concluded that BUB1 protects centromeric cohesion in mitosis through SGO1 (2). BUB1-mediated phosphorylation of CDC20 is required for proper checkpoint signaling and that inhibition of APC by BUB1 may partly account for the sensitivity of the spindle checkpoint. BUB1 kinase creates a mark for shugoshin localization and the correct partitioning of chromosomes.

References:

1. Leland, S.et.al: Heterozygosity for a Bub1 mutation causes female-specific germ cell aneuploidy in mice. Proc. Nat. Acad. Sci. 106: 12776-12781, 2009.

2. Tang, Z.et.al: Human Bub1 protects centromeric sister-chromatid cohesion through Shugoshin during mitosis. Proc. Nat. Acad. Sci. 101: 18012-18017, 2004.

Specificity:

Recognizes the BUB1protein

Cross Reactivity:

Human and Rat

Host / Isotype / Clone#:

Rabbit, IgG

Immunogen:

BUB1 antibody was raised against a 22 amino acid synthetic peptide from near the carboxy terminus of human BUB1

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Western blot analysis of bub1 in A-20 cell lysate with BUB1 antibody at (A) 1 and (B) 2 ug/ml.