Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
P64-63CR-100	100 ug	$455
P64-63CR-BULK	BULK	Contact Us

Overview:

Protein kinase C delta (PKCδ) is a member of the protein kinase C (PKC) family of serine-threonine kinases. It is a 79 kDa protein kinase that shows strict dependence on the presence of phospholipids, but shows no activation by Ca2+ (1). Phosphatidylinositol is the most potent activator of PKC delta. Northern blot analysis indicated that PKC delta is widely distributed in almost all the tissues and is a major isoform of PKC expressed in hemopoietic cells (2). PKC delta is involved in fundamental cellular functions regulated by diacylglycerols and mimicked by phorbol esters.

References:

1. Leibersperger, H. et al: Immunological demonstration of a calcium-unresponsive protein kinase C of the delta-type in different species and murine tissues. Predominance in epidermis. J Biol Chem. 1991 Aug 5; 266(22):14778-84. 2. Mischak, H. et al: Mouse protein kinase C-delta, the major isoform expressed in mouse hemopoietic cells: sequence of the cDNA, expression patterns, and characterization of the protein. Biochemistry. 1991 Aug 13; 30(32):7925-31.

Specificity:

Recognizes the PKCδ protein

Cross Reactivity:

Western blot human and mouse PKCd

PKCd; from other species may also be detectable.

Host :

Rabbit

Immunogen:

Synthesized peptide derived from C-terminus of human PKCδ protein

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Representative Western blot with Anti- PKCδ (1:1000) using 15ng of GST-tagged human recombinant PKCα, PKCβI, PKCβII, PKCγ, PKCδ, PKCε, PKCν, PKCθ, PKCμ, PKCυ, PKCζ, and PKCι.

Sample Data:

Representative Western blot (1:1000) with PKCδ using 10 μg, 30μg, 50μg of 293 cell lysates.

Product Datasheets

M191-2

Yang Weiwei et al., EGFR-Induced and PKCε Monoubiquitylation-Dependent NF-_KB Activation Upregulates PKM2 Expression and Promotes Tumorigenesis Molecular Cell November 2012 10.1016/j.molcel.2012.09.028

J Xue et al., Tumour suppressor TRIM33 targets nuclear [beta]-catenin degradation Nature Communications February 2015 10.1038/ncomms7156

E. McAllister Fiona et al., Correlation profiling for determining kinase-substrate relationships Methods March 2013 10.1016/j.ymeth.2013.03.012

M Tandon et al., SD-208, a novel protein kinase D inhibitor, blocks prostate cancer cell proliferation and tumor growth in vivo by inducing G2/M cell cycle arrest. PLoS One March 2015 10.1371/journal.pone.0119346

Sletten Torunn et al., Nucleolin Regulates Phosphorylation and Nuclear Export of Fibroblast Growth Factor 1 (FGF1) PLoS One March 2014 10.1371/journal.pone.0090687

Tandon Manuj et al., New Pyrazolopyrimidine Inhibitors of Protein Kinase D as Potent Anticancer Agents for Prostate Cancer Cells PLoS One September 2013 10.1371/journal.pone.0075601

J. Coultrap Steven et al., Improving a Natural CaMKII Inhibitor by Random and Rational Design PLoS One October 2011 10.1371/journal.pone.0025245

Decornez H?l?ne et al., Design, Selection, and Evaluation of a General Kinase-Focused Library ChemMedChem June 2009 10.1002/cmdc.200900164

Bhandaria Deepali et al., Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration?proliferation dichotomy PNAS July 2015 10.1073/pnas.1514157112

Anti-PKC delta

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