Recombinant full-length human MEK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. M02-10G
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| M02-10G-05 | 5 ug | $226 | |
| M02-10G-10 | 10 ug | $325 | |
| M02-10G-BULK | BULK | Contact Us |
Overview:
MEK1 is a member of the dual specificity protein kinase family that acts as a mitogen-activated protein kinase (MAPK) kinase. MEK1 lies upstream of MAPK/ERK and stimulates the enzymatic activity of MAPK/ERK upon a wide variety of extra- and intracellular signals. As an essential component of MAPK/ERK signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development (1). Constitutive activation of MEK1 results in cellular transformation. Thus, MEK1 represents a likely target for pharmacologic intervention in proliferative disease such as cancer (2).
Gene Aliases:
MAP2K1, MKK1, MAPKK1, PRKMK1
Genbank Number:
References:
1. Seger, R. et al: The MAPK signaling cascade. FASEB J. 9: 726-735, 1995.
2. Sebolt-Leopold, J S. et al: Blockade of the MAP kinase pathway suppresses growth of colon tumors in vivo. Nature Med. 5: 810-816, 1999.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
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Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
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Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~72 kDa
Spirli Carlo et al., Cyclic AMP/PKA-dependent paradoxical activation of Raf/MEK/ERK signaling in polycystin-2 defective mice treated with sorafenib Hepatology August 2012 10.1002/hep.25872
Yang Shuping et al., CDK1 Phosphorylation of YAP Promotes Mitotic Defects and Cell Motility and Is Essential for Neoplastic Transformation Cancer Research November 2013 10.1158/0008-5472.CAN-13-2049
Yanga Shuping et al., Phosphorylation of KIBRA by the extracellular signal-regulated kinase (ERK)?ribosomal S6 kinase (RSK) cascade modulates cell proliferation and migration Cellular Signaling February 2014 10.1016/j.cellsig.2013.11.012
Chao; Xu, Fengrong; Niu, Yan; Wu, Yun; Sun, Jing; Peng Wang et al., Synthesis and Biological Evaluations of 3-Benzothiazol-2-yl Coumarin Derivatives as MEK1 Inhibitors Letters in Drug Design & Discovery October 2013 10.2174/15701808113109990012
et al., Stem Cell Factor-Inducible MITF-M Expression in Therapeutics for Acquired Skin Hyperpigmentation Theranostics December 2022
et al., Perphenazine exerts antitumor effects on HUT78 cells through Akt dephosphorylation by protein phosphatase 2A Oncology Letters February 2021
et al., Purpurogallin is a novel mitogen-activated protein kinase kinase 1/2 inhibitor that suppresses esophageal squamous cell carcinoma growth in vitro and in vivo. Molecular carcinogenesis November 2019
et al., Discovery of carbazole derivatives as novel allosteric MEK inhibitors by pharmacophore modeling and virtual screening European Journal of Medicinal Chemistry September 2019
Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, Neurobiology, Ser/Thr Kinases
MEK1, Unactive, M02-14H
RAF1 (EE), Active, R01-13G
MEK2, Unactive, M03-14G
AMPK (A1/B2/G1), Active, P50-10H
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