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SRC, Active

Recombinant full-length Rous sarcoma virus SRC was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
Catalog No. S19-10G




Catalog No. Pack Size Price (USD)
S19-10G-05 5 ug $226
S19-10G-10 10 ug $325
S19-10G-BULK BULK Contact Us  


Overview:

SRC family belongs to non-receptor tyrosine kinases. SRC was originally identified as a transforming protein of the Rous sarcoma virus (RSV) that had enzymatic ability to phosphorylate tyrosine in protein substrates (1). SRC is overexpressed and activated in a large number of human malignancies and has been linked to the development of cancer and progression to distant metastases (2). In addition to increasing cell proliferation, a key role of SRC in cancer seems to be the ability to promote invasion and motility, functions that might contribute to tumour progression


Gene Aliases:

ASV; SRC1; c-SRC; p60-Src


Genbank Number:


References:


1. Collett, M S. et al: Protein kinase activity associated with the avian sarcoma virus src gene product. Proc Natl Acad Sci U S A. 1978 Apr;75(4):2021-4.

2. Jacobs, C. et al: Expression of pp60c-src protein kinase in adult and fetal human tissue: high activities in some sarcomas and mammary carcinomas. Cancer Res. 1983 Apr;43(4):1696-702.


Specific Activity:

Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Storage, Stability and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

~85 kDa



Product Datasheets



 J Liang et al., PKM2 dephosphorylation by Cdc25A promotes the Warburg effect and tumorigenesis. Nature Communications August 2016 10.1038/ncomms12431

 Keller N et al., Studies of the molecular mechanism of caspase-8 activation by solution NMR Cell Death and Differentiation April 2010 10.1038/cdd.2009.155

 Patela Priyank et al., Brk/Protein Tyrosine Kinase 6 Phosphorylates p27KIP1, Regulating the Activity of Cyclin D?Cyclin-Dependent Kinase 4 Molecular and Cellular Biology January 2015 10.1128/MCB.01206-14

 Gupta Shalini et al., Kinase-Actuated Immunoaggregation of Peptide-Conjugated Gold Nanoparticles Small June 2010 10.1002/smll.201000099

 Huang Yongqi et al., The Activity and Stability of the Intrinsically Disordered Cip/Kip Protein Family AreRegulated by Non-Receptor Tyrosine Kinases Journal of Molecular Biology January 2015 10.1016/j.jmb.2014.11.011

 Jun Cho Hee et al., EphrinB1 Interacts with CNK1 and Promotes Cell Migration through c-Jun N-terminal Kinase (JNK) Activation Journal of Biological Chemistry May 2014 10.1074/jbc.M114.558809

 Nama Sangkil et al., Novel synthetic derivatives of the natural product berbamine inhibit Jak2/Stat3 signaling and induce apoptosis of human melanoma cells Molecular Oncology October 2012 10.1016/j.molonc.2012.05.002

 E. Salhia Hussam et al., Cardiac troponin I tyrosine 26 phosphorylation decreases myofilament Ca 2+ sensitivity and accelerates deactivation Journal of Molecular and Cellular Cardiology September 2014 10.1016/j.yjmcc.2014.09.013

 Zhou Xi et al., The CHMP4b- and Src-docking sites in the Bro1 domain are autoinhibited in the native state of Alix Biochemical Journal March 2009 10.1042/BJ20081388

 Ghadiali JE et al., Protein Kinase-Actuated Resonance Energy Transfer in Quantum Dot-Peptide Conjugates ACS Nano July 2010 10.1021/nn101293s

 Beebe Kristin et al., Post-translational modification and conformational state of Heat Shock Protein 90 differentially affect binding of chemically diverse small molecule inhibitors Oncotarget July 2013 10.18632/oncotarget.1099

 Y Jin et al., Src drives the Warburg effect and therapy resistance by inactivating pyruvate dehydrogenase through tyrosine-289 phosphorylation. Oncotarget May 2016 10.18632/oncotarget.7159

 Figel Dwyer Sheila et al., Identification of Novel Focal Adhesion Kinase Substrates: Role for FAK in NFKB Signaling International Journal of Biological Sciences February 2015 10.7150/ijbs.10273

 Kosten Jonas et al., Efficient Modification of Alpha-Synuclein Serine 129 by Protein Kinase CK1 Requires Phosphorylation of Tyrosine 125 as a Priming Event ACS Chemical Neuroscience October 2014 10.1021/cn5002254

 Martic? Sanela et al., Electrochemical investigations into Tau protein phosphorylations Analyst March 2012 10.1039/c2an35097a

 Rao Suman et al., Target Modulation by a Kinase Inhibitor Engineered to Induce a Tandem Blockade of the Epidermal Growth Factor Receptor (EGFR) and c-Src: The Concept of Type III Combi-Targeting PLoS One February 2015 10.1371/journal.pone.0117215

 Martica Sanela et al., Probing copper/tau protein interactions electrochemically Analytical Biochemistry November 2013 10.1016/j.ab.2013.07.015

  et al., Discovery of Novel Small-Molecule Inhibitors of NF-?B Signaling with Antiinflammatory and Anticancer Properties. Journal of medicinal chemistry June 2019

  et al., Phosphorylation of human placental aromatase CYP19A1 The Biochemical journal February 2020

  et al., Analysis of EYA3 Phosphorylation by Src Kinase Identifies Residues Involved in Cell Proliferation International Journal of Molecular Sciences December 2019


PRODUCT RESOURCES
RESEARCH AREAS

Angiogenesis, Apoptosis/Autophagy, Cancer, Cytoplasmic Tyrosine Kinases, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, JAK/STAT Pathway


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