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WIP1, Active

Recombinant full-length human WIP1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. P04-20G


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Catalog No. Pack Size Price (USD)
P04-20G-05 5 ug $226
P04-20G-10 10 ug $325
P04-20G-BULK BULK Contact Us  


Overview:

Wild-type p53-induced phosphatase 1 (WIP1) is a member of the PP2C family of Ser/Thr protein, and is highly expressed in testis and ovary (1). The expression of WIP1 is induced in responses to the environmental stresses (2). WIP1 dephosphorylates CHK2 at Thr-68 in as a negative regulator in the response of DNA damage (2). WIP1 suppresses the DNA double strand break repair by dephosphorylating the phosphorylated Ser-139 in a histone variant gamma-H2AX (3).


Gene Aliases:

PPM1D; JDVS; IDDGIP; PP2C-DELTA


Genbank Number:


References:


1. Fiscella M. et. al: Wip1, a novel human protein phosphatase that is induced in response to ionizing radiation in a p53-dependent manner. PNAS. 1997 Jun 10; 94(12):6048-53.

2. Yoda A. et. al: Intrinsic kinase activity and SQ/TQ domain of Chk2 kinase as well as N-terminal domain of Wip1 phosphatase are required for regulation of Chk2 by Wip1. J Biol Chem. 2006 Aug 25; 281(34):24847-62.

3. Moon S. H. et. al: Wild-type p53-induced phosphatase 1 dephosphorylates histone variant gamma-H2AX and suppresses DNA double strand break repair. J Biol Chem. 2010 Apr 23; 285(17):12935-47.


Specific Activity:

Sample Activity Plot. For specific information on a given lot, see related technical data sheet.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Storage, Stability, and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

110 kDa



Product Datasheets



Safety Datasheet



There are no related publications available for this product.


RESEARCH AREAS

Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Inflammation, Neurobiology, NfkB Pathway, p38 Pathway, Ser/Thr Kinases


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