MLK2 or mixed-lineage kinase 2 is a member of the serine/threonine kinase family, which activates MAPK8 JNK and MKK4/SEK1. MLK2 itself can be phoshorylated, and activated by JNK kinases. MLK2 is also known as mitogen-activated protein kinase kinase kinase 10 (MAP3K10). MLK2 functions preferentially on the JNK signaling pathway which is involved in nerve growth factor (NGF) induced neuronal apoptosis. MLK2 was isolated as a cDNA fragment from MKN28 gastric cancer cell library using degenerate PCR (1). MLK2 is highly expressed in brain, skeletal muscle, and testis (2).
1. Katoh, M. et.al: Cloning and characterization of MST, a novel (putative) serine/threonine kinase with SH3 domain. Oncogene 10: 1447-1451, 1995.
2. Dorow, D. S. et.al: complete nucleotide sequence, expression, and chromosomal localisation of human mixed-lineage kinase 2. Europ. J. Biochem. 234: 492-500, 1995.
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Apoptosis/Autophagy, Cell Cycle, JNK/SAPK Pathway, Ser/Thr Kinases