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NEK9, Active

Recombinant human NEK9 (1-979; deleted fragment of 346-731) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. N11-11G


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Catalog No. Pack Size Price (USD)
N11-11G-05 5 ug $226
N11-11G-10 10 ug $325
N11-11G-BULK BULK Contact Us  


Overview:

NEK9 is a member of the NEK family and has high homology to NEK1, NEK3 and NEK4. NEK9 is activated during mitosis and binds specifically to RAN GTPase and is a substrate for CDC2 phosphorylation (1). NEK9 plays a role in the control of mitotic progression and is regulated by CDC2 and RAN GTPase. Overexpression of both active and inactive variants of NEK9 is toxic to cells and inhibits cell division causing abnormal nuclear morphologies. NEK9 can catalyze the phosphorylation of recombinant NEK6 and NEK7 in vitro leading to its activation. This suggests that NEK9 may be responsible for activation of NEK6 and NEK7 during mitosis (2).


Gene Aliases:

NEK8, NERCC, NERCC1, MGC16714, MGC138306, DKFZp434D0935


Genbank Number:


References:


1. Roig J, et al: Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression. Genes Dev. 16: 1640-1658, 2002.

2. Belham C, et al: A mitotic cascade of NIMA family kinases: Nercc1/Nek9 activates the Nek6 and Nek7 kinases. J. Biol. Chem. 278: 34897-34909, 2003.


Specific Activity:

Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Storage, Stability and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

~115 kDa



There are no related publications available for this product.


RESEARCH AREAS

Cancer, Cell Cycle, Ser/Thr Kinases


RELATED PRODUCTS


  NEK11, Active, N13-10G

  NEK7, Active, N09-10G

  NEK2, Active, N03-10G

  NEK3, Active, N05-10G


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