Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
A16-63R-100	100 ug	$270
A16-63R-BULK	BULK	Contact Us

Catalog No.

Pack Size

Price (USD)

A16-63R-100

100 ug

$270

A16-63R-BULK

BULK

Overview:

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473 (1).

References:

1. Anderson, KE. et al: Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91.

Specificity:

Recognizes the AKT1, AKT2, and AKT3 protein

Cross Reactivity:

Western blot AKT1, AKT2, and AKT3 from human and mouse cells

AKT1, AKT2, and AKT3 from other species may also be detectable.

Host:

Rabbit

Immunogen:

Synthetic peptide corresponding to amino acids 471-475 of human AKT

Purification:

Affinity chromatography

Stability:

Store at 4°C (add 0.1% NaN₃) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Representative western blot with Anti-AKT (pan) (1:1000) using 20 ng of human recombinant AKT1, AKT2, and AKT3.