CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain. Active form of CDK5, which has also been called neuronal cdc2-like kinase, is a heterodimer of CDK5 and a 25 kDa protein which is derived proteolytically from a 35 kDa brain and neuron-specific protein and is essential for the kinase activity of CDK5 (1). CDK5 has emerged as a crucial regulator of neuronal migration in the developing central nervous system. CDK5 phosphorylates a diverse list of substrates, implicating it in the regulation of a range of cellular processes - from adhesion and motility, to synaptic plasticity and drug addiction (2).
CDK5: PSSALRE p35: CDK5R1; CDK5P35, CDK5R, NCK5A, p35, p35nck5a
1. Tang, D. et al: Cyclin-dependent kinase 5 (Cdk5) and neuron-specific Cdk5 activators. Prog Cell Cycle Res. 1996;2:205-16.
2. Dhavan, R. et al: A decade of CDK5. Nat Rev Mol Cell Biol. 2001 Oct;2(10):749-59.
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
~59 kDa/~60 kDa
M Kanke et al., Cohesin acetylation and Wapl-Pds5 oppositely regulate translocation of cohesin along DNA. EMBO Journal November 2016 10.15252/embj.201695756
Chi TF et al., Cyclin-Dependent Kinase 5 (CDK5)-Mediated Phosphorylation of Upstream Stimulatory Factor 2 (USF2) Contributes to Carcinogenesis Cancers April 2019 10.3390/cancers11040523
Ko Yu et al., Site-specific phosphorylation of Fbxw7 by Cdk5/p25 and its resulting decreased stability are linked to glutamate-induced excitotoxicity. Cell Death & Disease August 2019 10.1038/s41419-019-1818-4
Rouget Rapha et al., Cyclin Dependent Kinase 5 Phosphorylation Of Familial Prion Protein Mutants Exacerbates Conversion Into Amyloid Structure. Journal of Biological Chemistry January 2015 10.1074/jbc.M114.630699
S. Desai Shruti et al., GSK-3β Protein Phosphorylates and Stabilizes HLXB9 Protein in Insulinoma Cells to Form a Targetable Mechanism of Controlling Insulinoma Cell Proliferation Journal of Biological Chemistry January 2014 10.1074/jbc.M113.533612
Wrasidlo Wolf et al., Neuroprotective effects of the anti-cancer drug sunitinib in models of HIV neurotoxicity suggests potential for the treatment of neurodegenerative disorders British Journal of Pharmacology December 2014 10.1111/bph.12875
BK Binukumar et al., Profiling of p5, a 24 Amino Acid Inhibitory Peptide Derived from the CDK5 Activator, p35 CDKR1 Against 70 Protein Kinases. Journal of Alzheimer's Disease September 2016 10.3233/JAD-160458
C Ianes et al., CK1δ activity is modulated by CDK2/E- and CDK5/p35-mediated phosphorylation Amino Acids February 2016 10.1007/s00726-015-2114-y
F Tian et al., The neuroprotective mechanism of puerarin in the treatment of acute spinal ischemia?reperfusion injury is linked to cyclin-dependent kinase 5 Neuroscience Letters September 2014 10.1016/j.neulet.2014.09.049
Bhandaria Deepali et al., Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration?proliferation dichotomy PNAS July 2015 10.1073/pnas.1514157112
Cancer, Cell Cycle, Ser/Thr Kinases