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CDK4/CyclinD1, Active

Recombinant full-length human CDK4 and CyclinD1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
Catalog No. C31-10G




Catalog No. Pack Size Price (USD)
C31-10G-05 5 ug $226
C31-10G-10 10 ug $325
C31-10G-BULK BULK Contact Us  


Overview:

CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of RB protein leading to an active transcriptional repression of E2F complex (1). CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2 (2).


Gene Aliases:

CDK4: CMM3; PSK-J3; MGC14458 CyclinD1: BCL1, PRAD1, U21B31, D11S287E


Genbank Number:


References:


1. Harbour, J W. et al: Cdk phosphorylation triggers sequential intramolecular interactions that progressively block Rb functions as cells move through G1. Cell 98: 859-869, 1999.

2. Matsuura, I. et al: Cyclin-dependent kinases regulate the antiproliferative function of Smads. Nature 430: 226-231, 2004.


Specific Activity:

Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Storage, Stability and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

~57 kDa and CyclinD1 ~61 kDa



 M Kanke et al., Cohesin acetylation and Wapl-Pds5 oppositely regulate translocation of cohesin along DNA. EMBO Journal November 2016 10.15252/embj.201695756

 Sankaranarayanan R et al., The Flavonoid Metabolite 2,4,6-Trihydroxybenzoic Acid Is a CDK Inhibitor and an Anti-Proliferative Agent: A Potential Role in Cancer Prevention. Cancers March 2019 10.3390/cancers11030427

 Rizzolio F et al., Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1 Cell Death & Differentiation July 2012 10.1038/cdd.2011.202

 Liu Haidan et al., A Chrysin Derivative Suppresses Skin Cancer Growth by Inhibiting Cyclin-dependent Kinases Journal of Biological Chemistry September 2013 10.1074/jbc.M113.464669

 Liu Shu et al., Structural and functional analysis of cyclin D1 reveals p27 and substrate inhibitor binding requirements ACS Chemical Biology August 2012 10.1021/cb1001262

 Sanphui Priyankar et al., Efficacy of Cyclin Dependent Kinase 4 Inhibitors as Potent Neuroprotective Agents against Insults Relevant to Alzheimer?s Disease PLoS One November 2013 10.1371/journal.pone.0078842

 Liu Lingling et al., Cyclin-Dependent Kinase 4 Phosphorylates and Positively Regulates PAX3-FOXO1 in Human Alveolar Rhabdomyosarcoma Cells PLoS One February 2013 10.1371/journal.pone.0058193

 Lucchetti C et al., The Prolyl Isomerase Pin1 Acts Synergistically with CDK2 to Regulate the Basal Activity of Estrogen Receptor ? in Breast Cancer PLoS One February 2013 10.1371/journal.pone.0055355

 Dachineni Rakesh et al., Salicylic acid metabolites and derivatives inhibit CDK activity: Novel insights into aspirin's chemopreventive effects against colorectal cancer Int J Oncol. October 2017 10.3892/ijo.2017.4167

 McInnes C et al., Cyclin based inhibitors of CDK2 and CDK4 Patent US9376465 B2 June 2016

 Bhandaria Deepali et al., Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration?proliferation dichotomy PNAS July 2015 10.1073/pnas.1514157112

  et al., Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production Cell reports January 2020

  et al., CDK4/6 Inhibition Augments Anti-Tumor Immunity by Enhancing T Cell Activation Cancer discovery February 2019

  et al., Mutant p53 Gains Its Function via c-Myc Activation upon CDK4 Phosphorylation at Serine 249 and Consequent PIN1 Binding Molecular cell December 2018

  et al., CDK6 protects epithelial ovarian cancer from platinum?induced death via FOXO3 regulation EMBO Molecular Medicine August 2017

  et al., Oncogenic Activity of Amplified Miniature Chromosome Maintenance 8 in Human Malignancies Oncogene March 2017

  et al., DNA damage in embryonic neural stem cell determines FTLDs’ fate via early-stage neuronal necrosis Life Science Alliance June 2021

  et al., Discovery of novel cyclin-dependent kinase (CDK) and histone deacetylase (HDAC) dual inhibitors with potent in vitro and in vivo anticancer activity. European journal of medicinal chemistry October 2020


PRODUCT RESOURCES
RESEARCH AREAS

Cancer, Cell Cycle, Ser/Thr Kinases


RELATED PRODUCTS


  CDK5, Unactive, C33-14G

  Rb (C-Term) Protein, R05-55G

  p21CIP1 Protein, C27-30G

  CDKN3 Protein, C01-34G


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