Recombinant full-length human CDK5 and p25 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
Catalog No. C33-10G
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| C33-10G-05 | 5 ug | $226 | |
| C33-10G-10 | 10 ug | $325 | |
| C33-10G-BULK | BULK | Contact Us |
Overview:
CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain. Active form of CDK5, which has also been called neuronal cdc2-like kinase, is a heterodimer of CDK5 and a 25 kDa protein which is derived proteolytically from a 35 kDa brain and neuron-specific protein and is essential for the kinase activity of CDK5 (1). CDK5 has emerged as a crucial regulator of neuronal migration in the developing central nervous system. CDK5 phosphorylates a diverse list of substrates, implicating it in the regulation of a range of cellular processes - from adhesion and motility, to synaptic plasticity and drug addiction (2).
Gene Aliases:
CDK5: PSSALRE p25: CDK5R1; CDK5P35, CDK5R, NCK5A, p23, p35, p35nck5a
References:
1. Tang, D. et al: Cyclin-dependent kinase 5 (Cdk5) and neuron-specific Cdk5 activators. Prog Cell Cycle Res. 1996;2:205-16.
2. Dhavan, R. et al: A decade of CDK5.
Nat Rev Mol Cell Biol. 2001 Oct;2(10):749-59.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
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Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
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Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~59 kDa and p25 ~49 kDa
Arif A et al., Multisite Phosphorylation of S6K1 Directs a Kinase Phospho-code that Determines Substrate Selection Molecular Cell February 2019 10.1016/j.molcel.2018.11.017
M Kanke et al., Cohesin acetylation and Wapl-Pds5 oppositely regulate translocation of cohesin along DNA. EMBO Journal November 2016 10.15252/embj.201695756
VA Cazares et al., Dynamic Partitioning of Synaptic Vesicle Pools by the SNARE-Binding Protein Tomosyn. Journal of Neuroscience November 2016 10.1523/JNEUROSCI.1297-16.2016
Miller Nimrod et al., Non-Aggregating Tau Phosphorylation by Cyclin-Dependent Kinase 5 Contributes to Motor Neuron Degeneration in Spinal Muscular Atrophy Journal of Neuroscience April 2015 10.1523/JNEUROSCI.3716-14.2015
S. Desai Shruti et al., GSK-3β Protein Phosphorylates and Stabilizes HLXB9 Protein in Insulinoma Cells to Form a Targetable Mechanism of Controlling Insulinoma Cell Proliferation Journal of Biological Chemistry January 2014 10.1074/jbc.M113.533612
BK Binukumar et al., Profiling of p5, a 24 Amino Acid Inhibitory Peptide Derived from the CDK5 Activator, p35 CDKR1 Against 70 Protein Kinases. Journal of Alzheimer's Disease September 2016 10.3233/JAD-160458
C Ianes et al., CK1δ activity is modulated by CDK2/E- and CDK5/p35-mediated phosphorylation Amino Acids February 2016 10.1007/s00726-015-2114-y
F Tian et al., The neuroprotective mechanism of puerarin in the treatment of acute spinal ischemia?reperfusion injury is linked to cyclin-dependent kinase 5 Neuroscience Letters September 2014 10.1016/j.neulet.2014.09.049
Bhandaria Deepali et al., Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration?proliferation dichotomy PNAS July 2015 10.1073/pnas.1514157112
et al., A network pharmacology-based study on key pharmacological pathways and targets of Qi Fu Yin acting on Alzheimer's disease Experimental Gerontology July 2021
et al., Cyclin-dependent kinase 1 (CDK1)-mediated mitotic phosphorylation of the transcriptional co-repressor Vgll4 inhibits its tumor-suppressing activity The Journal of Biological Chemistry September 2017
Cancer, Cell Cycle, Ser/Thr Kinases
CDK9, Unactive, C40-14G
CDK2/CyclinE1, Unactive, C29-14G
CDK8, Unactive, C38-14G
Anti-CDC25A, C04-63R
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