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CK1 epsilon (R178C), Active

Full length recombinant human CK1epsilon (R178C) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. C66-12BG


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Catalog No. Pack Size Price (USD)
C66-12BG-05 5 ug $226
C66-12BG-10 10 ug $325
C66-12BG-BULK BULK Contact Us  


Overview:

CK1epsilon is a member of the CK1 family of serine/threonine protein kinases which play an important role in diverse cell processes, including DNA replication and repair. CK1epsilon is a regulator of Yes-associated protein (YAP) transcription coactivator which is a key regulator of organ size and a candidate human oncogene. CK1 epsilon is activated by CCK2R and this then phosphorylates PKD2 at Ser244. Phosphorylation of PKD2 leads to its nuclear accumulation and efficient phosphorylation of nuclear PKD2 substrates in human gastric cancer cells (1). CKI epsilon can phosphorylate topoisomerase (topo) IIalpha at serine-1106 and this regulates the enzyme activity and sensitivity to topo II-targeted drugs (2).


Gene Aliases:

CSNK1E, HCKIE, MGC10398


Genbank Number:


References:


1. von Blume J. et al: Phosphorylation at Ser244 by CK1 determines nuclear localization and substrate targeting of PKD2. EMBO J. 2007 Nov 14;26(22):4619-33.

2. Grozav, A G. et al: Casein kinase I delta/epsilon phosphorylates topoisomerase IIalpha at serine-1106 and modulates DNA cleavage activity. Nucleic Acids Res. 2009 Feb;37(2):382-92.


Specific Activity:

Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Storage, Stability, and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

~72 kDa



RESEARCH AREAS

Cancer, Inflammation, Neurobiology, NfkB Pathway, Ser/Thr Kinases, WNT Signaling


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