Recombinant full-length human CDK1 and CyclinB1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
Catalog No. C22-10G
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CDK1 or Cell Division Control protein 1 is essential for the completion of START, the controlling event in the cell cycle that is required to initiate mitosis. CDK1 is a catalytic subunit of a protein kinase complex, called the M-Phase Promoting Factor that induces entry into mitosis and is universal among eukaryotes (1). Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer has also been demonstrated (2).
CDK1: CDC2 CyclinB1: CCNB1, CCNB
1. Vantieghem, A. et al: Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. J. Biol. Chem. 2002; 277(40):37718-31.
2. Rigas, A.C. et al: Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer. Oncogene. 2007; 18.
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
~58 kDa and CyclinB1 ~75 kDa
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M Kanke et al., Cohesin acetylation and Wapl-Pds5 oppositely regulate translocation of cohesin along DNA. EMBO Journal November 2016 10.15252/embj.201695756
Sankaranarayanan R et al., The Flavonoid Metabolite 2,4,6-Trihydroxybenzoic Acid Is a CDK Inhibitor and an Anti-Proliferative Agent: A Potential Role in Cancer Prevention. Cancers March 2019 10.3390/cancers11030427
Yin L et al., Cyclin-dependent kinase 1-mediated phosphorylation of SET at serine 7 is essential for its oncogenic activity Cell Death & Disease May 2019 10.1038/s41419-019-1621-2
Suenaga Yusuke et al., NCYM, a Cis-Antisense Gene of MYCN, Encodes a De Novo Evolved Protein That Inhibits GSK3? Resulting in the Stabilization of MYCN in Human Neuroblastomas PLoS Genetics January 2014 10.1371/journal.pgen.1003996
Yang Shuping et al., CDK1 Phosphorylation of YAP Promotes Mitotic Defects and Cell Motility and Is Essential for Neoplastic Transformation Cancer Research November 2013 10.1158/0008-5472.CAN-13-2049
S Stauffer et al., CDK1-mediated mitotic phosphorylation of PBK is involved in cytokinesis and inhibits its oncogenic activity Cell signal November 2017 10.1016/j.cellsig.2017.08.001
X Chen et al., MST2 phosphorylation at serine 385 in mitosis inhibits its tumor suppressing activity Cell Signal December 2016 10.1016/j.cellsig.2016.08.013
X Chen et al., Ajuba Phosphorylation by CDK1 Promotes Cell Proliferation and Tumorigenesis. Journal of Biological Chemistry July 2016 10.1074/jbc.M116.722751
Ji Ming et al., Phospho-regulation of KIBRA by CDK1 and CDC14 phosphatase controls cell-cycle progression Biochemical Journal October 2012 10.1042/BJ20120751
Zhao Yulei et al., Regulation of sensitivity of tumor cells to antitubulin drugs by Cdk1-TAZ signalling Oncotarget May 2015 10.18632/oncotarget.4259
Moa Min et al., Phosphorylation of Xenopus p31comet potentiates mitotic checkpoint exit Cell Cycle April 2015 10.1080/15384101.2015.1033590
M Lawson et al., Synthesis, biological evaluation and molecular modeling studies of imidazo[1,2-a]pyridines derivatives as protein kinase inhibitors. European Journal of Medicinal Chemistry November 2016 10.1016/j.ejmech.2016.07.040
Dachineni Rakesh et al., Salicylic acid metabolites and derivatives inhibit CDK activity: Novel insights into aspirin's chemopreventive effects against colorectal cancer Int J Oncol. October 2017 10.3892/ijo.2017.4167
Bhandaria Deepali et al., Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration?proliferation dichotomy PNAS July 2015 10.1073/pnas.1514157112
Cancer, Cell Cycle, Ser/Thr Kinases