Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
P03-63BR-100	100 ug	$595
P03-63BR-BULK	BULK	Contact Us

Catalog No.

Pack Size

Price (USD)

P03-63BR-100

100 ug

$595

P03-63BR-BULK

BULK

Overview:

PAK proteins is a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK2 is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell (1). The CDC42 and RAC1 induce autophosphorylation of PAK2, which stimulates sustained phosphorylation of other substrates (2).

References:

1. Koeppel, M. A. et.al: Identification and characterization of PS-GAP as a novel regulator of caspase-activated PAK-2. J. Biol. Chem. 279: 53653-53664, 2004.

2. Martin, G. A. et.al: A novel serine kinase activated by rac1/CDC42Hs-dependent autophosphorylation is related to PAK65 and STE20. EMBO J. 14: 1970-1978, 1995.

Specificity:

Recognizes the PAK2 protein

Cross Reactivity:

Human, Mouse and Rat

Host / Isotype / Clone#:

Rabbit, IgG

Immunogen:

PAK2 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human PAK2

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Western blot analysis of PAK2 in Jurkat lysate with PAK2 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.