HSP27 is a member of heat shock proteins (HSPs) which are synthesized in cells in response to heat shock and other metabolic stresses and provide a transient state of thermotolerance. HSP27 plays a major role in the increased thermal resistance acquired by cells after exposure to HSP inducers (1). The level of HSP27 phosphorylation is significantly elevated after exposure of cells to heat shock, sodium arsenite, IL-1 and TNF-. MAPKAPK2 and MAPKAPK3 are both activated by these conditions and can phosphorylate HSP27 on serine residues (2).
CMT2F; HSPB1; HSP28; Hsp25; HS.76067; DKFZp586P1322
1. Landry, J. et al: Heat shock resistance conferred by expression of the human HSP27 gene in rodent cells. J Cell Biol. 1989 Jul;109(1):7-15.
2. Clifton, A D. et al: A comparison of the substrate specificity of MAPKAP kinase-2 and MAPKAP kinase-3 and their activation by cytokines and cellular stress. FEBS Lett. 1996 Sep 2;392(3):209-14.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Kostenko Sergiy et al., The diterpenoid alkaloid noroxoaconitine is a Mapkap kinase 5 (MK5/PRAK) inhibitor Cellular and Molecular Life Sciences July 2011 10.1007/s00018-010-0452-1
Yalcin Abdullah et al., Nuclear Targeting of 6-Phosphofructo-2-kinase (PFKFB3) Increases Proliferation via Cyclin-dependent Kinases* Journal of Biological Chemistry November 2012 10.1074/jbc.M109.016816
Kostenko Sergiy et al., PKA-induced F-actin rearrangement requires phosphorylation of Hsp27 by the MAPKAP kinase MK5 Cellular Signaling May 2009 10.1016/j.cellsig.2009.01.009
Shiryaev A et al., Distinct roles of MK2 and MK5 in cAMP/PKA-and stress/p38MAPK-induced heat shock protein 27 phosphorylation Journal of Molecular Signaling May 2011 10.1186/1750-2187-6-4
Kostenko Sergiy et al., Phosphorylation of heat shock protein 40 (Hsp40/DnaJB1) by mitogen-activated protein kinase-activated protein kinase 5 (MK5/PRAK) The International Journal of Biochemistry & Cell Biology February 2014 10.1016/j.biocel.2013.11.004
Cancer, Cellular Stress, p38 Pathway