Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
A16-60M-25	25 ug	$95
A16-60M-100	100 ug	$375
A16-60M-BULK	BULK	Contact Us

Overview:

AKT1/PKBα is a serine/threonine kinase that belongs to the AKT family. AKT1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). AKT1 is frequently overexpressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas and prostate as well as lymphomas and leukemias (2).

References:

1. Coffer, PJ. et al: Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem J. 1998 Oct 1; 335 (Pt 1): 1-13.

2. Anderson, KE. et al: Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91.

Specificity:

Anti-AKT1 antibody recognizes the AKT protein.

Cross Reactivity:

Western blot AKT1 from human and mouse cells

AKT from other species may also be detectable.

Host / Isotype / Clone#:

Mouse, Monoclonal, 9A4

Immunogen:

Recombinant full length active AKT1 protein

Purification:

Affinity purified using Protein G

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Representative western blot (1:2500) using 10µg, 20µg, and 40µg of HEK-293 cell lysate.

Product Datasheets

V058-2

K3337-1

Ghosh Dastidar Somasish et al., FoxG1 Promotes the Survival of Postmitotic Neurons Journal of Neuroscience January 2011 10.1523/JNEUROSCI.2897-10.2011

Cena Bo et al., The Pim-1 protein kinase is an important regulator of MET receptor tyrosine kinase levels and signaling Molecular and Cellular Biology March 2014 10.1128/MCB.00147-14

Lamouille Samy et al., TGF-?-induced activation of mTOR complex 2 drives epithelial?mesenchymal transition and cell invasion Journal of Cell Science March 2012 10.1242/jcs.095299

Zenga in et al., Lanthionine synthetase C-like protein 2 (LanCL2) is a novel regulator of Akt Molecular Biology of the Cell September 2014 10.1091/mbc.E14-01-0004

Cennia Vittoria et al., Ankrd2/ARPP is a novel Akt2 specific substrate and regulates myogenic differentiation upon cellular exposure to H2O2 Molecular Biology of the Cell July 2011 10.1091/mbc.E10-11-0928

Bavelloni Alberto et al., Prohibitin 2 represents a novel nuclear AKT substrate during all-trans retinoic acid-induced differentiation of acute promyelocytic leukemia cells FASEB Journal February 2014 10.1096/fj.13-244368

Pecorari Luisa et al., Elongation Factor 1 alpha interacts with phospho-Akt in breast cancer cells and regulates their proliferation, survival and motility Molecular Cancer August 2009 10.1186/1476-4598-8-58

M. Singel Stina et al., KIF14 Promotes AKT Phosphorylation and Contributes to Chemoresistance in Triple-Negative Breast Cancer Neoplasia March 2014 10.1016/j.neo.2014.03.008

Treebak Jonas et al., Identification of a novel phosphorylation site on TBC1D4 regulated by AMP-activated protein kinase in skeletal muscle American Journal of Physiology - Cell Physiology November 2009 10.1152/ajpcell.00297.2009

SC Stolze et al., Photo-crosslinking of clinically relevant kinases using H89-derived photo-affinity probes Molecular BioSystems May 2016 10.1039/c6mb00257a

Asakura Keisuke et al., The Cytostatic Effects of Lovastatin on ACC-MESO-1 Cells Journal of Surgical Research October 2011 10.1016/j.jss.2011.06.037

Anti-AKT1

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