Recombinant full-length human AMPK (combination of A1/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using C-terminal His tags.
Catalog No. P47-10H
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AMPK is a heterotrimer protein kinase consisting of an α catalytic subunit, and non-catalytic β and γ subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status (1). In response to cellular metabolic stresses, AMPK is activated and phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating biosynthesis of fatty acid and cholesterol (2).
Subunit A1: PRKAA1, MGC33776, MGC57364 Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785 Subunit G1: PRKAG1, AMPKG, MGC8666
1. Minokoshi, Y. et al:AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus. Nature 428: 569-574, 2004.
2. Hardie, D G. et al: The AMP-activated protein kinase--fuel gauge of the mammalian cell Eur J Biochem. 1997 Jun 1;246(2):259-73.
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
~68kDa (A1), ~38kDa (B1), ~40kDa (G1)
Guha P et al., IPMK Mediates Activation of ULK Signaling and Transcriptional Regulation of Autophagy Linked to Liver Inflammation and Regeneration Cell Reports March 2019 10.1016/j.celrep.2019.02.013
Shuai Z et al., Cellular energy stress induces AMPK-mediated regulation of glioblastoma cell proliferation by PIKE-A phosphorylation Cell death & disease March 2019 10.1038/s41419-019-1452-1
Liu X et al., AMPK-mediated degradation of Nav1. 5 through autophagy The FASEB Journal April 2019 10.1096/fj.201801583RR
PM Anders et al., The KSHV K1 Protein Modulates AMPK Function to Enhance Cell Survival. PLoS Pathogens November 2016 10.1371/journal.ppat.1005985
G Mahapatra et al., Phosphorylation of Cytochrome c Threonine 28 Regulates Electron Transport Chain Activity in Kidney: Implications for AMP Kinase. Journal of Biological Chemistry October 2016 10.1074/jbc.M116.744664
Meltzer-Mats Ella et al., Synthesis and Mechanism of Hypoglycemic Activity of Benzothiazole Derivatives Journal of Medicinal Chemistry June 2013 10.1021/jm4001488
Choi Jiwon et al., Sanguinarine is an allosteric activator of AMP-activated protein kinase Biochemical and Biophysical Research Communications September 2011 10.1016/j.bbrc.2011.08.081
L. Figarola James et al., Small?molecule COH-SR4 inhibits adipocyte differentiation via AMPK activation International Journal of Molecular Medicine March 2013 10.3892/ijmm.2013.1313
B Eric et al., Discovery of TBC1D1 as an Insulin-, AICAR-, and Contraction-stimulated Signaling Nexus in Mouse Skeletal Muscle Journal of Biological Chemistry April 2008 10.1074/jbc.M708839200
Apoptosis/Autophagy, Cancer, Cellular Stress, Metabolic Disorder, Ser/Thr Kinases