The 2019-nCoV N-S1 Human IgM ELISA Kit is an enzyme-linked immunosorbent assay for the panspecific
detection of Human anti-2019-nCoV N Protein & Spike Protein S1 IgM in serum or other
biological samples. Based on indirect ELISA principles, the 2019-nCoV N-S1 Human IgA ELISA
employs recombinant 2019-nCoV N Protein & Spike Protein S1 RBD Chimera coated on a 96-
well plate. Controls and samples (with necessary dilution) are pipetted into the wells; the
target IgM in the controls and samples binds to the immobilized protein chimera. The intensity of color development in the wells is proportional to the amount of target IgM bound.
SignalChem Cat# |
Component Name |
Size |
Storage |
WB20-09 | Wash Buffer, 20X | 15 ml x 2 vials | Store kit and contents at 4oC for up to 6 months. Store unused multi well strips in the provided resealable bag and use within one month after opening. |
CP04-C19NSD | 2019-nCoV RBD-N Protein Chimera Coated Plate | 1 plate |
C19NS1M-POS | nCoV N-S1 IgM POS Control | 1 ml x 1 vial | |
C19NS1M-NEG | nCoV N-S1 IgM NEG Control | 1 ml x 1 vial |
SS01-09 | Stop Solution | 6 ml x 1 vial |
E02-09 | nCoV Ig Sample Dilution Buffer | 10 ml x 2 vials |
CS02-506 | One-Step TMB Substrate | 10 ml x 1 vial |
H38M-63DG | Anti-Human IgM Fc Detection Antibody, 1X | 10 ml x 1 vial |
1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.
2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30.
3. Long Q, et al: Antibody responses to SARS-CoV-2 in patients with COVID-19 Quan-Xin Long. Nature Medicine. 2020, 29 April.
4. Ma H, et al: Serum IgA, IgM, and IgG responses in COVID-19. Cell Mol Immunol. 2020, 17: 773-
Calculation and interpretation of assay results
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