The anti-nCoV S1 Human IgG-IgA-IgM ELISA Kit is an enzyme-linked immunosorbent assay for the
pan-specific detection of Human anti-nCoV Spike protein S1 IgG-IgA-IgM in serum or other biological
samples. Based on indirect ELISA principles, the anti-nCoV S1 Human IgG-IgA-IgM ELISA employs
recombinant nCoV Spike protein RBD coated on a 96-well plate. Controls and samples (with necessary
dilution) are pipetted into the wells; the target IgG-IgA-IgM in the controls and samples binds to
the immobilized Spike protein. The intensity of color development in the wells is proportional to the amount of target IgG-IgA-IgM bound.
SignalChem Cat# |
Component Name |
Size |
Storage |
WB20-09 | Wash Buffer, 20X | 15 ml x 2 vials | Store kit and contents at 4oC for up to 6 months. Store unused multi well strips in the provided resealable bag and use within one month after opening. |
C19S1P-POS | IgG-IgA-IgM Positive Control | 1 ml x 1 vial | |
C19S1P-NEG | IgG-IgA-IgM Negative Control | 1 ml x 1 vial |
SS01-09 | Stop Solution | 6 ml x 1 vial |
E02-09 | nCoV Ig Sample Dilution Buffer | 10 ml x 2 vials |
CS02-506 | One-Step TMB Substrate | 10 ml x 1 vial |
CP03-C19SD | 2019-nCoV Spike Protein RBD Coated Plate | 1 plate |
H38P-63DG | Anti-Human IgG-IgA-IgM Fc Detection Antibody, 1X | 10 ml x 1 vial |
1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin.
Nature. 2020, 579:270-89.
2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30.
3. Long Q, et al: Antibody responses to SARS-CoV-2 in patients with COVID-19 Quan-Xin Long. Nature Medicine. 2020, 29 April.
4. Ma H, et al: Serum IgA, IgM, and IgG responses in COVID-19. Cell Mol Immunol. 2020, 17: 773-775.
Calculation and interpretation of assay results
Name |
Catalog Number |
Species |
Tag |
Expression System |
Sequence |
Genbank Number |
2019-nCoV Spike protein RBD, Fc Tag | C19SD-G241F | SARS-CoV-2 | Fc | CHO cells | 319-541 | MN908947 |
2019-nCoV Spike protein RBD, HIS Tag | C19SD-G241H | SARS-CoV-2 | HIS | CHO cells | 319-541 | MN908947 |
2019-nCoV Spike protein S1, Fc Tag | C19S1-G241F | SARS-CoV-2 | Fc | CHO cells | 16-685 | MN908947 |
2019-nCoV Spike protein S1, HIS Tag | C19S1-G241H | SARS-CoV-2 | HIS | CHO cells | 16-685 | MN908947 |
2019-nCoV Spike protein S2, Fc Tag | C19S2-G241F | SARS-CoV-2 | Fc | CHO cells | 686-1212 | MN908947 |
Anti 2019-nCoV Spike Protein mIgG Antibody | C19S1-61MH | Human Fab – Mouse IgG Fc | | CHO cells | | |
Anti 2019-nCoV Spike Protein hIgG Antibody, HRP conjugated | C19S1-60DH | Human, IgG1 | | CHO cells | | |
Anti-Human IgG Antibody mIgM, Unconjugated | H38-60M | Mouse, IgM | | | | |
Anti 2019-nCoV N-Protein hIgG Antibody, HRP conjugated | C19NP-60DH | Human, IgG1 | | CHO cells | | |
2019-nCoV S1 Protein ELISA Kit | C19SD-876 | | | | | |
2019-nCoV S1 Human IgG ELISA Kit | C19S1-877 | | | | | |
2019-nCoV S1 Human IgA ELISA Kit | C19S1-A877 | | | | | |
2019-nCoV N-S1 Human IgA ELISA Kit | C19NS1-A877 | | | | | |
2019-nCoV N-S1 Human IgG ELISA Kit | C19NS1-877 | | | | | |
2019-nCoV N-S1 Human IgG-IgA-IgM ELISA Kit | C19NS1-P877 | | | | | |
2019-nCoV Neutralization ELISA Kit | C19SD-N887 | | | | | |
2019-nCoV High Sensitivity S1 Protein ELISA Kit | C19S1-876 | | | | | |
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