The 2019-nCoV High Sensitivity S1 Protein ELISA Kit v2.0 is a sandwich enzyme-linked
immunosorbent assay for the quantitative measurement of 2019-nCoV Coronavirus Spike
protein. The S1 Protein ELISA employs a capture anti-S1 protein antibody coated on a 96-well
plate. Calibrators and samples are pipetted into the wells; the target protein in the calibrators and
samples binds to the immobilized capture antibody. The wells are washed and the HRP-labeled
Detection antibody is then added. After washing away the unbound HRP-antibodies, a colorimetric
substrate solution (TMB) is pipetted to the wells. The absorbance of the color at 450 nm is
measured. The intensity of color development in the wells is proportional to the amount of target
protein bound.
SignalChem Cat# |
Component Name |
Size |
Storage |
C19S1-HS61DH | Anti-nCoV S1 Detection Antibody, HS-1X | 10 ml x 1 vial | Store kit and contents at 4oC for up to 6 months. Store unused multi well strips in the provided resealable bag and use within one month after opening. |
CP06-642HL | Anti 2019-nCoV Spike Protein Coated Plate | 1 plate | |
CS02-506 | One-Step TMB Substrate | 10 ml x 1 vial |
E01-09 | nCoV ELISA Sample Dilution Buffer | 10 ml x 2 vials |
SS01-09 | Stop Solution | 6 ml x 1 vial |
C19SD-CAL50 | Calibrator 50 | 1 ml x 1 vial |
WB20-09 | Wash Buffer, 20X | 15 ml x 2 vials |
1.Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin.
Nature. 2020, 579:270-89.
2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30.
3. Lan J, et al: Crystal structure of the 2019-nCoV Spike receptor-binding domain bound with the ACE2
receptor. bioRxiv. doi: https://doi.org/10.1101/2020.02.19.956235.
The 2019-nCoV Spike protein concentration of a biological sample may be calculated using the
Calibrator by generating a standard curve that correlates the measured O.D. values.
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