The 2019-nCoV N-S1 Human IgG ELISA Kit is an enzyme-linked immunosorbent assay for the panspecific
detection of Human anti-2019-nCoV N Protein & Spike Protein S1 IgG in serum or other
biological samples. Based on indirect ELISA principles, the 2019-nCoV N-S1 Human IgG
ELISA employs recombinant 2019-nCoV N Protein & Spike Protein S1 RBD Chimera coated on
a 96-well plate. Controls and samples (with necessary dilution) are pipetted into the wells; the
target IgG in the controls and samples binds to the immobilized protein chimera. The intensity of color development in the wells is proportional to the amount of target IgG bound.
SignalChem Cat# |
Component Name |
Size |
Storage |
WB20-09 | Wash Buffer, 20X | 15 ml x 2 vials | |
C19NS1-POS | nCoV N-S1 IgG POS Control | 1 ml x 1 vial |
C19NS1-NEG | nCoV N-S1 IgG NEG Control | 1 ml x 1 vial |
SS01-09 | Stop Solution | 6 ml x 1 vial |
E02-09 | nCoV Ig Sample Dilution Buffer | 10 ml x 2 vials |
CS02-506 | One-Step TMB Substrate | 10 ml x 1 vial |
H38-63DG | Anti-Human IgG Fab Detection Ab | 10 ml x 1 vial |
CP04-C19NSD | 2019-nCoV RBD-N Protein Chimera Coated Plate | 1 plate | Store kit and contents at 4oC for up to 6 months. Store unused multi well strips in the provided resealable bag and use within one month after opening. |
1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.
2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30.
3. Long Q, et al: Antibody responses to SARS-CoV-2 in patients with COVID-19 Quan-Xin Long. Nature Medicine. 2020, 29 April.
4. Ma H, et al: Serum IgA, IgM, and IgG responses in COVID-19. Cell Mol Immunol. 2020, 17: 773-775
Calculation and interpretation of assay results
Name |
Catalog Number |
Species |
Tag |
Expression System |
Sequence |
Genbank Number |
2019-nCoV Spike protein RBD, Fc Tag | C19SD-G241F | SARS-CoV-2 | Fc | CHO cells | 319-541 | MN908947 |
2019-nCoV Spike protein RBD, HIS Tag | C19SD-G241H | SARS-CoV-2 | HIS | CHO cells | 319-541 | MN908947 |
2019-nCoV Spike protein S1, Fc Tag | C19S1-G241F | SARS-CoV-2 | Fc | CHO cells | 16-685 | MN908947 |
2019-nCoV Spike protein S1, HIS Tag | C19S1-G241H | SARS-CoV-2 | HIS | CHO cells | 16-685 | MN908947 |
2019-nCoV Spike protein S2, Fc Tag | C19S2-G241F | SARS-CoV-2 | Fc | CHO cells | 686-1212 | MN908947 |
Anti 2019-nCoV Spike Protein hIgG Antibody, HRP conjugated | C19S1-60DH | Human, IgG1 | | CHO cells | | |
Anti 2019-nCoV Spike Protein mIgG Antibody | C19S1-61MH | Human Fab – Mouse IgG Fc | | CHO cells | | |
Anti 2019-nCoV Spike Protein hIgA Antibody | C19S1-A60H | Human, IgA | | CHO cells | | |
Anti 2019-nCoV N-Protein hIgG Antibody, HRP conjugated | C19NP-60DH | Human, IgG1 | | CHO cells | | |
2019-nCoV S1 Protein ELISA Kit | C19SD-876 | | | | | |
2019-nCoV S1 Human IgG ELISA Kit | C19S1-877 | | | | | |
2019-nCoV S1 Human IgG-IgA-IgM ELISA Kit | C19S1-P877 | | | | | |
2019-nCoV N-S1 Human IgM ELISA Kit | C19NS1-M877 | | | | | |
2019-nCoV N-S1 Human IgG-IgA-IgM ELISA Kit | C19NS1-P877 | | | | | |
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