Specificity:

Recognizes the longest isoform of the ATG5 protein

Cross Reactivity:

Human, Mouse and Rat

Host / Isotype / Clone#:

Rabbit, IgG

Immunogen:

ATG5 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ATG5.

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Western blot analysis of ATG5 in rat spleen tissue lysate with Anti-ATG5 at (A) 2 ug/mL and (B) 2 ug/mL.

Sample Data:

Immunohistochemistry of ATG5 in human spleen tissue with Anti-ATG5 at 2.5 ug/mL.

Sample Data:

Immunofluorescence of ATG5 in Human Spleen cells with Anti-ATG5 at 20 ug/mL.

References :

1. Gozuacik D and Kimchi A, Autophagy as a cell death and tumor suppressor mechanism. Oncogene 2004; 23:2891-906.

2. Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.

3. Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol.2000; 150:1507-13.

4. Hanada T, Noda NN, Satomi Y, et al. The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy. J. Biol. Chem.2007; 282:37298-302.