Rabbit Polyclonal Antibody
Catalog No. C28-362R
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
C28-362R-100 | 100 ug | $369 | |
C28-362R-BULK | BULK | Contact Us |
Overview:
KIP1 (cyclin-dependent kinase inhibitor 1B) is a kinesin-related motor protein required for mitotic spindle assembly and chromosome segregation (1). Many tumorigenic processes modulate cell-cycle progression by regulating the levels of the cyclin-dependent kinase inhibitor KIP1. KIP1 binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (2). The phosphorylation- and ubiquitination-dependent proteolysis of KIP1 is implicated in control of the G1-S transition in the cell cycle. KIP1 is critical for retinoblastoma protein (Rb)-induced cellular proliferative senescence.
References:
1. Hoyt, M A. et al: Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly. J. Cell Biol. 1992 118(1):109-120.
2. Carrano,A C. et al: SKP2 is required for ubiquitin-mediated degradation of the CDK inhibitor p27. Nat. Cell Biol. 1999; 1, 193-199
Specificity :
Recognizes the p27 protein from human tissue
Cross Reactivity:
Human
Host Isotype / Clone#:
Rabbit, Antiserum
Immunogen:
The antibody was produced against full length recombinant human p27 protein
Purification:
Delipidation and defibrination
Stability:
1yr at –20oC from date of shipment
Sample Data:
Western blot using Anti-p27KIP1 (CDKN1B) (1:500 dilution) shows detection of p27 protein in MCF7 whole cell lysate (lanes 1-3) (arrowhead).
M Kanke et al., Cohesin acetylation and Wapl-Pds5 oppositely regulate translocation of cohesin along DNA. EMBO Journal November 2016 10.15252/embj.201695756
Yalcin Abdullah et al., Nuclear Targeting of 6-Phosphofructo-2-kinase (PFKFB3) Increases Proliferation via Cyclin-dependent Kinases* Journal of Biological Chemistry November 2012 10.1074/jbc.M109.016816
Cancer, Cell Cycle
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