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PAD1 siRNA Set I

PAD1 is a pool of three individual synthetic siRNA duplexes designed to knock-down human PAD1 mRNA expression. Each siRNA is 19-25 bases in length.
Catalog No. P312-911


For enquiry on all siRNA Sets email us at orders@signalchem.com


Catalog No. Pack Size Price (USD)
P312-911-05 5 nmol $655
P312-911-20 20 nmol $1205
P312-911-50 50 nmol $2085
P312-911-BULK BULK Contact Us  


Overview:

PAD1 is a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. PAD1 is a component of the 26S proteasome, a multiprotein complex that degrades proteins targeted for destruction by the ubiquitin pathway. The overexpression of PAD1 induced a distinctive pattern of multidrug resistance in mammalian cells and moderate resistance to ultraviolet light (1). PAD1 is also responsible for substrate deubiquitination during proteasomal degradation (2).


Gene Aliases:

HPAD10; PADI1; PDI; PDI1


Genbank Number:


References:


1. Spataro, V. et.al: Resistance to diverse drugs and ultraviolet light conferred by overexpression of a novel human 26 S proteasome subunit. J. Biol. Chem. 272: 30470-30475, 1997.

2. Yao, T. et.al: A cryptic protease couples deubiquitination and degradation by the proteasome. Nature 419: 403-407, 2002.


Specificity:

PAD1 siRNAs are designed to specifically knock-down human PAD1 expression.


Reconstitution Protocol:

Each siRNA duplex is supplied as 2.5nmol per tube (for example 6 x 2.5nmol = 3 x 5nmol per duplex). Briefly centrifuge one tube per duplex (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend each 2.5nmol tube in 50 ul of DEPC-treated water (included in kit), which results in a 1x stock solution (50 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.


Storage and Stability:

The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Format:

Lyophilized powder



Product Datasheets



There are no related publications available for this product.


RESEARCH AREAS

Inflammation


RELATED PRODUCTS


  PAD Detection Kit, P312-863

  PAD4 Inhibitor Screening Kit, P310DG-863

  PAD3 Inhibitor Screening Kit, P310CG-863

  PAD2 (mouse) Inhibitor Screening Kit, P310BG-863


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