Mouse Monoclonal Antibody
Catalog No. R46-61M
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
R46-61M-100 | 100 ug | $248 | |
R46-61M-BULK | BULK | Contact Us |
Overview:
RFP or red fluorescent protein from sea anemone Entacmaea quadricolor is a member of the green florescent protein family and is extensively used as a tag and reporter protein in molecular and cellular biology. RFP has a molecular weight of approximately 29 kDa and an excitation and emission maxima at 559 and 611 nm (1). RFP fluoresces over a wide pH range (4-10) with high quantum yield. Like other anthozoan fluorescent proteins, RFP forms tetramers at physiological concentrations.
References:
1. Wiedenmann, J . et al: Red fluorescent protein eqFP611 and its genetically engineered dimeric variants. J Biomed Opt. 2005 Jan-Feb;10(1):14003..
Specificity:
Recognizes native and denatured forms of RFP protein.
Cross Reactivity:
Western blot RFP and its variants tag-RFP, DeRed, mCherry and mOrange, etc
Other RFP variants may also be detectable.
Host / Isotype / Clone#:
Mouse, IgG1, RF5R
Immunogen:
RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugates
Purification:
Affinity chromatography
Stability:
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data:
Representative western blot with Anti-RFP (1:1000) using 5 μl, 10 μl and 20 μl of 293 cell lysate transfected with a RFP protein.
Eskelin K et al., Ribosome profiles and riboproteomes of healthy and potato virus A and Agrobacterium -infected Nicotiana benthamiana plants Molecular Plant Pathology October 2018 10.1111/mpp.12764
A Hafr et al., Formation of Potato Virus A-Induced RNA Granules and Viral Translation Are Interrelated Processes Required for Optimal Virus Accumulation. PLoS Pathogens December 2015 10.1371/journal.ppat.1005314
Christine Ramel Marie et al., The ventral to dorsal BMP activity gradient in the early zebrafish embryo is determined by graded expression of BMP ligands Developmental Biology March 2013 10.1016/j.ydbio.2013.03.003
et al., The potyviral silencing suppressor HCPro recruits and employs host ARGONAUTE1 in pro-viral functions PLoS Pathogens October 2020
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