Rabbit Polyclonal Antibody
Catalog No. H87-63R
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
H87-63R-100 | 100 ug | $455 | |
H87-63R-BULK | BULK | Contact Us |
Overview:
HDAC5 or Histone deacetylase 5 belongs to the class II histone deacetylase/acuc/apha family that possesses histone deacetylase activity and represses transcription when tethered to a promoter (1). HDAC 5 plays a critical role in transcriptional regulation, cell cycle progression, and developmental events and also acts as a potential therapeutic target for the prevention of atherosclerosis. HDAC5 can co-immunoprecipitates with HDAC3 family members forming multicomplex proteins. HDAC5 can also interact with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. HDAC5 gene is thought to be associated with colon cancer (2).
References:
1. Grozinger, C. M. et.al : Three proteins define a class of human histone deacetylases related to yeast Hda1p. Proc. Nat. Acad. Sci. 96: 4868-4873, 1999.
2. Scanlan, M. J. et.al: Characterization of human colon cancer antigens recognized by autologous antibodies. Int. J. Cancer 76: 652-658, 1998.
Specificity:
Recognizes the human HDAC5 protein
Cross Reactivity:
Only a weak reaction with human HDAC4 in Western Blot.
HDAC5 from other species may also be detectable.
Host:
Rabbit
Immunogen:
Synthetic peptide corresponding to c-terminus of human HDAC5 conjugated to KLH.
Purification:
Affinity Chromatography
Stability:
Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data:
Representative western blot with Anti-HDAC5 (1:1000) using 25 ng of human recombinant proteins HDAC1 to HDAC11.
M. Mihaylova Maria et al., Class IIa Histone Deacetylases Are Hormone-Activated Regulators of FOXO and Mammalian Glucose Homeostasis Cell May 2011 10.1016/j.cell.2011.03.043
Duan Wenwen et al., Design, Synthesis and Antitumor Evaluation of Novel Histone Deacetylase (HDAC) Inhibitors Equipped with Phenylsulfonylfuroxan Module as Nitric Oxide (NO) Donor Journal of Medicinal Chemistry May 2015 10.1021/acs.jmedchem.5b00317
Hou Xuben et al., Enhancing the Sensitivity of Pharmacophore-Based Virtual Screening by Incorporating Customized ZBG Features: A Case Study Using Histone Deacetylase 8 Journal of Chemical Information and Modeling March 2015 10.1021/ci500762z
J. Evans Nathan et al., Detection of Lysine Deacetylase Activities : Bioluminescent Assays Offer Distinct Advantages over Fluorescent Approaches Genetic Engineering & Biotechnology News October 2011 10.1089/gen.31.19.13
Cardiovascular Disease, WNT Signaling
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