Recombinant full-length human CDK2 and CyclinE1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
Catalog No. C29-18G
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
C29-18G-05 | 5 ug | $226 | |
C29-18G-10 | 10 ug | $325 | |
C29-18G-BULK | BULK | Contact Us |
Overview:
CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including Cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B) (1). CDK2 phosphorylates multiple cellular substrates including SMAD3 and FOXO1. Phosphorylation of FOXO1 leads to its inhibition (2).
Gene Aliases:
CDK2: p33 (CDK2) CyclinE1: CCNE1, CCNE
References:
1. Levkau, B. et al: Cleavage of p21(Cip1/Waf1) and p27(Kip1) mediates apoptosis in endothelial cells through activation of Cdk2: role of a caspase cascade. Molec. Cell1: 553-563, 1998.
2. Huang, H. et al: CDK2-dependent phosphorylation of FOXO1 as an apoptotic response to DNA damage. Science314: 294-297, 2006.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~58kDa and CyclinE1 ~73kDa
M Kanke et al., Cohesin acetylation and Wapl-Pds5 oppositely regulate translocation of cohesin along DNA. EMBO Journal November 2016 10.15252/embj.201695756
X Chen et al., MST2 phosphorylation at serine 385 in mitosis inhibits its tumor suppressing activity Cell Signal December 2016 10.1016/j.cellsig.2016.08.013
Bhandaria Deepali et al., Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration?proliferation dichotomy PNAS July 2015 10.1073/pnas.1514157112
et al., Discovery of covalent CDK14 inhibitors with pan-TAIRE family specificity Cell chemical biology June 2020
et al., LIMD1 phosphorylation in mitosis is required for mitotic progression and its tumor-suppressing activity. The FEBS journal December 2019
Cancer, Cell Cycle, Ser/Thr Kinases
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