Recombinant full-length human CDK1 and CyclinB1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
Catalog No. C22B-10G
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| C22B-10G-05 | 5 ug | $226 | |
| C22B-10G-10 | 10 ug | $325 | |
| C22B-10G-BULK | BULK | Contact Us |
Overview:
CDK1 or Cell Division Control protein 1 is essential for the completion of START, the controlling event in the cell cycle that is required to initiate mitosis. CDK1 is a catalytic subunit of a protein kinase complex, called the M-Phase Promoting Factor that induces entry into mitosis and is universal among eukaryotes (1). Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer has also been demonstrated (2).
References:
1. Vantieghem, A. et al: Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. J. Biol. Chem. 2002; 277(40):37718-31.
2. Rigas, A.C. et al: Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer. Oncogene. 2007; 18.
Specific Activity:
Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
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Purity:
Sample Purity. For specific information on a given lot, see related technical data sheet
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Alternative Name(s):
CDK1: CDC2 CyclinB1: CCNB1, CCNB
Storage and Stability:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
CDK1 observed MW ~58 kDa.
CDK1 calculated MW ~60 kDa.
CyclinB1 observed MW ~75 kDa.
CyclinB1 calculated MW ~75 kDa.
There are no related publications available for this product.
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