Overview:

The CRBN/DDB1/CUL4A/RBX1 complex, also known as CRL4 CRBN E3 ubiquitin ligase complex, forms an E3 ubiquitin ligase that tags target proteins for ubiquitin-mediated degradation, thereby regulating various cellular processes such as cell cycle progression, DNA repair, and protein homeostasis. CRBN is a substrate receptor that serves as a binding site for specific target proteins, allowing the complex to recognize and ubiquitinate them for degradation. DDB1 acts as an adaptor protein that connects CRBN to CUL4A within the complex. CUL4 acts as a scaffold within the complex, providing the structural backbone for the assembly of the complex and facilitates the transfer of ubiquitin molecules to the target proteins. RBX1 interacts with CUL4A and facilitates the transfer of ubiquitin moieties to the target proteins, marking them for degradation by the proteasome.

Genbank Number:

CRBN: Q96SW2
DDB1: Q16531
CUL4A: Q13619
RBX1: P62877

References:

1. Ito T, et al. (2010). Identification of a primary target of thalidomide teratogenicity. Science, 327(5971), 1345-1350.
2. Sugasawa K, et al. (2005). A multistep damage recognition mechanism for global genomic nucleotide excision repair. Genes & Development, 19(17), 2192-2203.