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Anti-His

Rabbit Polyclonal Antibody
Catalog No. H99-63R


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Catalog No. Pack Size Price (USD)
H99-63R-25 25 ug $105
H99-63R-100 100 ug $248
H99-63R-BULK BULK Contact Us  


Overview:

This polyhistidine epitope tag is generally comprised of six consecutive histidine amino acid residues located at the N-terminal, C-terminal, or internally [1]. The 6 His-Tag is widely used because of its affinity to bind nickel or cobalt metal ions attached to sepharose, which can then be used to purify the protein in a native or denatured state [2].

References:


1. Jonathan, W. et al: Epitope tagging, Annual Review of Genetics, 1998; 32, 601-618

2. Schmeisser, H, et al:, Binding Characteristics of IFN-alpha Subvariants to IFNAR2-EC and Influence of the 6-Histidine Tag. J Interferon Cytokine Res. 2006; Dec. 26(12):866-76.


Specificity:

Anti-His antibody recognizes the N-terminal, C-terminal or internal 6X His-tagged fusion protein


Species Cross Reactivity:

All proteins tagged with 6X His


Host:

Rabbit


Purification:

Purified by antigen-specific affinity chromatography


Stability:

Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.


Sample Data:

Representative western blot (1:1000) using in a positive multi-tag cell lysate that contains a 52 kDa His tagged fusion protein.



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