Rabbit Polyclonal Antibody
Catalog No. L95-67CR
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
L95-67CR-100 | 100 ug | $565 | |
L95-67CR-BULK | BULK | Contact Us |
Overview:
Post-translational modification of proteins and peptides is a robust way to regulate function of existing proteins or peptides. Methylation on lysine residues is one example of post-translational modification and is performed by a variety of protein methyl transferases in the cell. C/EBPs are extensively modified by methylation of lysine side chains and this regulated methylation profoundly affects the activity of C/EBPs (1). The Methylated Lysine Antibody conjugated to FITC detects methylation on lysine residues in proteins and peptides.
References:
1. Leutz, R. et al: Crosstalk between phosphorylation and multi-site arginine/lysine methylation in C/EBPs. Transcr. 2011 Jan;2(1):3-8.
Specificity:
Recognizes proteins with tri-methylation on lysine residues (N-epsilon).
Cross Reactivity:
Pan-specific antibody. No cross-reactions detected with acetylated, mono-methylated and di-methylated proteins.
Host:
Rabbit
Immunogen:
Methylated lysine-KLH conjugates.
Purification:
By affinity chromatography on a tri-methyl lysine agarose column
Stability:
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data:
Representative immunofluorescent stain with Anti-Methylated Lysine (Tri- epsilon-N-methyl), FITC Conjugated (1:1000) using the paraformaldehyde fixed human melanoma cells (MMRU).
There are no related publications available for this product.
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