Recombinant 2019-nCoV RNA-dependent RNA polymerase (RdRp) was expressed in E. coli cells using a C-terminal His tag.
Catalog No. C19RP-G241H
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
C19RP-G241H-05 | 5 ug | $226 | |
C19RP-G241H-10 | 10 ug | $325 | |
C19RP-G241H-20 | 20 ug | $649 | |
C19RP-G241H-50 | 50 ug | $1367 | |
C19RP-G241H-BULK | BULK | Contact Us |
Overview:
A novel coronavirus (SARS-CoV-2) has caused a global COVID-19 pandemic of respiratory disease in 2020 (1). RNA-dependent RNA polymerase (RdRp) is an enzyme that is crucial to life cycle of RNA viruses including coronaviruses (2). RdRp catalyzes the synthesis of viral RNA, thereby playing a central role in the replication and transcription cycle of COVID-19 viruses, possibly in complex with nsp7 and nsp8 (3). Therefore, RdRp is a prime target for nucleotide analog antiviral inhibitors, such as remedesivir, for development of new antiviral therapeutics (4).
Gene Aliases:
nsp12
Genbank Number:
References:
1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89
2. Subissi L, et al: One severe acute respiratory syndrome coronavirus protein complex integrates processive RNA polymerase and exonuclease activities. Proc. Natl. Acad. Sci. U.S.A. 2014, 111: E3900–E3909.
3. Gao Y, et al: Structure of the RNA-dependent RNA polymerase from COVID-19 virus. Science. 2020, 368: 779-782.
4. Wang M, et al: Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro. Cell Res. 2020, 30:269–271.
Activity:
Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~100 kDa
There are no related publications available for this product.
Acute Respiratory Distress Syndrome , Cardiovascular Disease, Cell Cycle, Cellular Stress, COVID19, Gastrointestinal Diseases , Infectious Diseases , Inflammation, Lung Diseases , Neurobiology, severe acute respiratory syndrome coronavirus 2 , Virology
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