Home   >    Proteins   >   Protein Kinases

CDK4/CyclinD3, Active

Recombinant full-length human CDK4 and CyclinD3 were co-expressed by baculovirus in Sf9 cells using an N-terminal GST tag on both proteins.
Catalog No. C31-18G


Shop Online



Catalog No. Pack Size Price (USD)
C31-18G-05 5 ug $226
C31-18G-10 10 ug $325
C31-18G-BULK BULK Contact Us  


Overview:

CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex (1). CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2 (2).


Gene Aliases:

CDK4: CMM3; PSK-J3; MGC14458 Cyclin D3: CCND3


Genbank Number:


References:


1. Harbour, J W. et al: Cdk phosphorylation triggers sequential intramolecular interactions that progressively block Rb functions as cells move through G1. Cell 98: 859-869, 1999.

2. Matsuura, I. et al: Cyclin-dependent kinases regulate the antiproliferative function of Smads. Nature 430: 226-231, 2004.


Specific Activity:

Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Storage, Stability and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

~58 kDa and CyclinD3 ~58 kDa



RESEARCH AREAS

Cancer, Cell Cycle, Ser/Thr Kinases


RELATED PRODUCTS


  p27KIP1 Protein, C28-30G

  Kinase Dilution Buffer III, K23-09

  Rb (C-Term) Protein, R05-55G

  CyclinE1 Protein, C82-30G


SIGNALCHEM BIOTECH INC.


About

Terms of Service

Privacy Policy

Get the latest resources, updates and offers in your inbox