Recombinant full-length human SMAD2 was expressed in E. coli cells using an N-terminal GST tag.
Catalog No. S11-30G
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
S11-30G-20 | 20 ug | $215 | |
S11-30G-50 | 50 ug | $435 | |
S11-30G-BULK | BULK | Contact Us |
Overview:
SMADs are essential intracellular components for the signal transduction of TGFβ family members. SMAD2 is an intracellular mediator of TGFβ family and activin type 1 receptor (1). SMAD2 mediate TGFβ signaling to regulate cell growth and differentiation. SMAD2 is released from cytoplasmic retention by TGFβ receptor-mediated phosphorylation. The phosphorylated SMAD2 then forms a heterodimeric complex with SMAD4, and this complex translocates from cytoplasm into nucleus. By interacting with DNA-binding proteins, SMAD2 complexes then positively or negatively regulate the transcription of target genes. Inactivating mutations in SMAD2 have been found in various cancers (2).
Gene Aliases:
JV18, MADH2, MADR2, JV18-1, hMAD-2, hSMAD2, MGC22139, MGC34440
Genbank Number:
References:
1. Masayuki, F. et al: Identification and Characterization of Constitutively Active Smad2 Mutants: Evaluation of Formation of Smad Complex and Subcellular Distribution. Molecular Endocrinol. 2000; 14 (10): 1583-1591.
2. Eppert, K. et al: MADR2 maps to 18q21 and encodes a TGF-beta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma. Cell. 1996; 86: 543-552.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~90 kDa
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