NEK11 is a member of the NEK family and the protein localizes to the nucleoli where it may function with NEK2A in the S-phase checkpoint. NEK2 phosphorylates NEK11 in its C-terminal autoinhibitory domain and elevates NEK11 kinase activity by dissociating the autoinhibitory domain from the N-terminal catalytic domain (1). NEK11 protein appears to play a role in DNA replication and response to genotoxic stress. NEK11 activity is activated in HeLa cells treated with various DNA-damaging agents and replication inhibitors. Activation of NEK11 by genotoxic agents is suppressed by inhibitor of ATR/ATM-mediated signalling indicating that NEK11 has a role in S-phase checkpoint downstream of ATR/ATM signalling (2).
1. Noguchi K, et al: Nucleolar Nek11 is a novel target of Nek2A in G1/S-arrested cells. J. Biol. Chem. 279: 32716-32727, 2004.
2. Noguchi K, et al: Nek11, a new member of the NIMA family of kinases, involved in DNA replication and genotoxic stress responses. J. Biol. Chem. 277: 39655-39665, 2002.
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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Cancer, Cell Cycle, Ser/Thr Kinases