Recombinant full-length human METTL12 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. M332-30G
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
M332-30G-05 | 5 ug | $259 | |
M332-30G-10 | 10 ug | $380 | |
M332-30G-BULK | BULK | Contact Us |
Overview:
Human methyltransferase like proteins (METTL) are part of a large protein family characterized by the presence of binding domains for S-adenosyl methionine, a co-substrate for methylation reactions (1). The methyltransferase-like protein 12 (METTL12) is localized in the mitochondrial matrix and methylates the lysine-368 that is localized near the active sites of citrate synthase (CS) (2). In response to alterations in metabolite levels, the METTL12-mediated methylation regulates the CS activity (3). Possible functions for the methylation of Lys-368 are in controlling substrate channeling itself, or in influencing protein–protein interactions in the metabolon (2).
Gene Aliases:
CS-KMT; METTL12; U99HG
Genbank Number:
References:
1. Ignatova, V.V. et al: The interactome of a family of potential methyltransferases in HeLa cells. Scientific reports. 2019; 9.1: 1-9. 2. Rhein, V.F. et al: Human METTL12 is a mitochondrial methyltransferase that modifies citrate synthase. FEBS letters. 2017; 591.12: 1641-1652. 3. Malecki, J. et al: Uncovering human METTL12 as a mitochondrial methyltransferase that modulates citrate synthase activity through metabolite-sensitive lysine methylation. Journal of Biological Chemistry. 2017; 292.43: 17950-17962.
Specific Activity:
Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity. For specific information on a given lot, see related technical data sheet.
Storage and Stability:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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Cell Cycle
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