Recombinant full-length human AMPK (combination of A2/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tags.
Catalog No. P48-10H
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
P48-10H-05 | 5 ug | $435 | |
P48-10H-10 | 10 ug | $655 | |
P48-10H-BULK | BULK | Contact Us |
Overview:
AMPK (A2/B1/G1) plays a key role in insulin signaling pathway and is a major therapeutic target for the treatment of diabetes (1). AMPK is viewed as a fuel sensor for glucose and lipid metabolism by modulating the activity of the autonomous nervous system in vivo. Short-term overexpression of a constitutively active form of AMPK in the liver leads to mild hypoglycemia and fatty liver due to increased fatty acid utilization (2).
Gene Aliases:
Subunits A2: PRKAA2, AMPK, AMPK2, PRKAA Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785 Subunit G1: PRKAG1, AMPKG, MGC8666
References:
1. Viollet, B. et al: Physiological role of AMP-activated protein kinase (AMPK): insights from knockout mouse models. Biochem. Soc. Trans. 2003; 31; 216–219.
2. Foretz, M. et al: Short-term overexpression of a constitutively active form of AMP-activated protein kinase in the liver leads to mild hypoglycemia and fatty liver. Diabetes, 2005; 54 (5);1331-1339.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~69kDa (A2), ~38kDa (B1), and ~40kDa (G1).
Q Zhu et al., Adipocyte-specific deletion of Ip6k1 reduces diet-induced obesity by enhancing AMPK-mediated thermogenesis. Journal of Clinical Investigation November 2016 10.1172/JCI85510
PM Anders et al., The KSHV K1 Protein Modulates AMPK Function to Enhance Cell Survival. PLoS Pathogens November 2016 10.1371/journal.ppat.1005985
Choi Jiwon et al., Sanguinarine is an allosteric activator of AMP-activated protein kinase Biochemical and Biophysical Research Communications September 2011 10.1016/j.bbrc.2011.08.081
B Eric et al., Discovery of TBC1D1 as an Insulin-, AICAR-, and Contraction-stimulated Signaling Nexus in Mouse Skeletal Muscle Journal of Biological Chemistry April 2008 10.1074/jbc.M708839200
et al., A Functional Signature Ontology (FUSION) screen detects an AMPK inhibitor with selective toxicity toward human colon tumor cells Scientific Reports February 2018
et al., The Journal of Biological Chemistry The Journal of Biological Chemistry August 2017 10.1074/jbc.M117.780874
Apoptosis/Autophagy, Cancer, Cellular Stress, Metabolic Disorder, Ser/Thr Kinases
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