Rabbit Polyclonal Antibody
Catalog No. C49-565R
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
C49-565R-100 | 100 ul | $675 | |
C49-565R-BULK | BULK | Contact Us |
Overview:
Members of the cAMP responsive element binding protein (CREB) family are critical mediators of gene expression in response to extracellular signals. Thus, they are essential regulators of adaptive behavior and long-term memory formation (1). A variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs), and Ca2+/calmodulin dependent protein kinases (CAMKs) phosphorylate CRE1B at Ser133, which is required for CREB-mediated transcription (2,3). Activation of CREB1 has been implicated in the survival of mammalian cells. Mice that do not express CREB in the central nervous system display extensive apoptosis of postmitotic neurons during development (4). CREB can bind promoters containing CRE and activator protein 1 (AP-1) sites either as homo- and heterodimers to control gene expression.
References:
1. Valverde, O. et al: Modulation of anxiety-like behavior and morphine dependene in CREB-deficient mice. Neuropsychopharmacology. 2004 Jun;29(6):1122-33.
2. Johannessen, M. et al: What turns CREB on? Cellular Signalling 2004 16:1211-1227.
3. Kornhauser, J M. et al: CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events. Neuron 2002 34:221-233.
4. Mantamadiotis, T. et al: Disruption of CREB function in brain leads to neurodegeneration. Nat genet. 2002 May; 31(1): 47-54.
Specificity:
Recognizes the CREB1 protein phosphorylated at serine 133
Cross Reactivity:
Human, Mouse, Rat, Bovine, Canine, Chicken, non-Human Primate, Sheep, Xenopus and Zebrafish
Host:
Rabbit, IgG
Immunogen:
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser383 conjugated to KLH
Purification:
Affinity Chromatography
Stability:
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data:
Western blot of forskolin stimulated rat hippocampal lysate showing specific immunolabeling of the ~45kDa CREB1 phosphorylated at Ser133 (Left lane). Phosphospecificity is shown in the right lane where the signal is completely eliminated by treatment with lambda phosphatase (lambda-Ptase, 1200 units for 30 min).
Sample Data:
Immunostaining of cultured rat hippocampal slices showing nuclear labeling of CREB phosphorylated at Ser133 in brown.
Sehgal Lalit et al., 14-3-3γ-mediated transport of plakoglobin to the cell border is required for the initiation of desmosome assembly in vitro and in vivo Cell Science May 2014 10.1242/jcs.125807
Peng Cong et al., Tumor Necrosis Factor Receptor-associated Factor Family Protein 2 Is a Key Mediator of the Epidermal Growth Factor-induced Ribosomal S6 Kinase 2/cAMP-responsive Element-binding Protein/Fos Protein Signaling Pathway Journal of Biological Chemistry July 2012 10.1074/jbc.M112.359521
Apoptosis/Autophagy, Cardiovascular Disease, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, PKA/PKC Pathway
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