Overview:

ZAP70 is a non-receptor protein tyrosine kinase (part of the Syk/Zap70 family) that is involved in signaling by the T-cell antigen receptor (TCR). Ligation of the TCR/CD3 receptor in Jurkat T-cells induces phosphoprotein complexes which contain ZAP70 (1). TCR zeta chains are initially phosphorylated by p56Lck that lead to the recruitment of ZAP70 via its SH2 domain. ZAP70 in turn phosphorylates other proteins in the TCR-phosphoprotein complex. One of the natural substrates for ZAP70 is the zeta-chain dimer of the TCR/CD3 complex (2).

References:

1. Duplay, P. et al: p56lck interacts via its src homology 2 domain with the ZAP-70 kinase. J Exp Med. 1994 Apr 1;179(4):1163-72.

2. Chan, A C. et al: Differential expression of ZAP-70 and Syk protein tyrosine kinases, and the role of this family of protein tyrosine kinases in TCR signaling. J Immunol. 1994 May 15;152(10):4758-66.

3. Salomon AR, et al. (2003) Proc Natl Acad Sci U S A; 100(2): 443-448

4. Ku GM, et al. (2001) EMBO J; 20(3): 457-465.

5. Tang J, et al. (1999) Proc Natl Acad Sci U S A; 96(17): 9775-9780.

6. Zhao Q, et al. (1996) Mol Cell Biol; 16(12): 6765-6774. 7. Williams BL, et al. (1999) EMBO J; 18(7): 1832-1844.