Recombinant full-length human MAPKAPK5 was expressed by E. coli cells using an N-terminal His tag.
Catalog No. M42-14BH
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
M42-14BH-20 | 20 ug | $215 | |
M42-14BH-50 | 50 ug | $435 | |
M42-14BH-BULK | BULK | Contact Us |
Overview:
MAPKAPK5 is a member of the serine/threonine kinase family that responds to cellular stress and proinflammatory cytokines. MAPKAPK5 is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta (1). MAPKAPK5 is activated in HeLa cells in response to cellular stress and proinflammatory cytokines. MAPKAPK5 activity is regulated by p38-alpha and p38-beta both in vitro and in vivo, and thr-182 is the regulatory phosphorylation site of MAPKAPK5 (2). In vitro, MAPKAPK5 kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites.
Genbank Number:
References:
1. New, L.; Jiang, Y. et al: PRAK, a novel protein kinase regulated by the p38 MAP kinase. EMBO J. 17: 3372-3384, 1998.
2. Ni, H.; Wang. et al: MAPKAPK5, a novel mitogen-activated protein kinase (MAPK)-activated protein kinase, is a substrate of the extracellular-regulated kinase (ERK) and p38 kinase. Biochem. Biophys. Res. Commun. 243: 492-496, 1998.
Alternative Name(s):
PRAK
Storage and Stability:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity:
Sample purity data. For lot-specific data, please refer to the respective datasheet
Molecular Weight:
Observed MW ~56 kDa. ; Calculated MW ~54 kDa
There are no related publications available for this product.
Ser/Thr Kinases
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