Recombinant full-length human p110alpha (E545K) mutant subunit and full-length human p85alpha wild-type subunit were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
Catalog No. P27-152G
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
P27-152G-05 | 5 ug | $259 | |
P27-152G-10 | 10 ug | $380 | |
P27-152G-BULK | BULK | Contact Us |
Overview:
"The PI3K comprises of a 110 kDa catalytic subunit and an 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110 alpha catalytic subunit (PIK3CA) is frequently mutated or amplified in a variety of cancers including ovarian and colon and this protein is one of the PI3K mutants (1). PIK3CA gene copy number is increased in over 30% of ovarian cancers and this leads to increased PI3-kinase activity. Furthermore, the activity of p110 alpha is essential for vascular development and inactivation of p110alpha leads to severe defects in angiogenic sprouting and vascular remodeling (2). "
References:
1. Samuels, Y. et al: High frequency of mutations of the PIK3CA gene in human cancers. Science 304: 554 only, 2004.
2. Graupera, M.et al: Angiogenesis selectively requires the p110-alpha isoform of PI3K to control endothelial cell migration. Nature 453: 662-666, 2008.
Alternative Name(s):
p110alpha: PIK3CA, MGC142161, MGC142163, p110-alpha p85-alpha: PIK3R1, GRB1, p85-ALPHA
Storage and Stability:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Specific Activity:
Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Molecular Weight:
p110α(E545K):
Calculated MW ~148 kDa.
p85α:
Calculated MW ~108 kDa.
There are no related publications available for this product.
Cancer, Cell Cycle, Receptor Tyrosine Kinases
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