Recombinant full-length human MEKK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. M11-10G
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
M11-10G-05 | 5 ug | $226 | |
M11-10G-10 | 10 ug | $325 | |
M11-10G-BULK | BULK | Contact Us |
Overview:
MEKK3 is a member of the MEKK family of protein kinases that directly regulate SAPK and ERK pathway but not the p38 pathway. MEKK3 modulates SAPK and ERK activity by activating the upstream targets SEK and MEK1/2, respectively (1). KSR2 can regulate the activity of MEKK3 and can reduce MEKK3-mediated ERK activation. Knockout of the MEKK3 gene in mice leads to disruption of blood vessel development and the structural integrity of the yolk sac (2). The blockage of angiogenesis in these mice is not a result of decreased VEGF1 expression but rather due to an intrinsic defect in Mekk3 -/- endothelial cells. Thus, MEKK3 is necessary for blood vessel development and may be a possible target for drugs that control angiogenesis.
Gene Aliases:
MAP3K3, MAPKKK3
Genbank Number:
References:
1. Ellinger-Ziegelbauer, H. Et al: Direct activation of the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by an inducible mitogen-activated protein kinase/ERK kinase kinase 3 (MEKK) derivative. J. Biol. Chem. 272: 2668-2674, 1997.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~105 kDa
Natsuo Takeda Armelle et al., Ubiquitin-dependent regulation of MEKK2/3-MEK5-ERK5 signaling module by XIAP and cIAP1 EMBO Journal August 2014 10.15252/embj.201487808
et al., Ripretinib (DCC-2618) Is a Switch Control Kinase Inhibitor of a Broad Spectrum of Oncogenic and Drug-Resistant KIT and PDGFRA Variants. Cancer cell February 2020
et al., Regulation of endothelial cell survival and death by the MAP kinase/ERK kinase kinase 3 - glyceraldehyde-3-phosphate dehydrogenase signaling axis. Cellular signalling July 2020
Apoptosis/Autophagy, ERK/MAPK Pathway, Invasion/Metastasis, JNK/SAPK Pathway, p38 Pathway, Ser/Thr Kinases
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