Recombinant full-length human AMPK (a combination of A1/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using N-terminal GST and C-terminal His tags.
Catalog No. P47-110GH
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
P47-110GH-05 | 5 ug | $435 | |
P47-110GH-10 | 10 ug | $655 | |
P47-110GH-BULK | BULK | Contact Us |
Overview:
AMPK is a heterotrimer protein kinase consisting of a α catalytic subunit, and non-catalytic β and γ subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status (1). In response to cellular metabolic stresses, AMPK is activated and phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating biosynthesis of fatty acid and cholesterol (2).
Gene Aliases:
Subunit A1: PRKAA1, MGC33776, MGC57364 Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785 Subunit G1: PRKAG1, AMPKG, MGC8666
References:
1. Minokoshi, Y. et al:AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus. Nature 428: 569-574, 2004.
2. Hardie, D G. et al: The AMP-activated protein kinase--fuel gauge of the mammalian cells Eur J Biochem. 1997 Jun 1;246(2):259-73.
Specific Activity:
Sample Activity Plot. For specific information on a given lot, see the related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see the related technical data sheet.
Storage, Stability, and Shipping:
Store product at –70oC. For optimal storage, aliquot targets into smaller quantities after centrifugation and store at the recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
approx. MW 94~100 kDa (A1), ~64 kDa (B1), and ~68 kDa (G1).
There are no related publications available for this product.
Apoptosis/Autophagy, Cancer, Cellular Stress, Metabolic Disorder, Ser/Thr Kinases
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