Recombinant human CDK12 (658-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. C62-35G
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
C62-35G-20 | 20 ug | $215 | |
C62-35G-50 | 50 ug | $435 | |
C62-35G-BULK | BULK | Contact Us |
Overview:
CDK12 forms a protein complex with cyclin K and this complex acts at the level of RNA polymerase II (RNAPII) to regulate expression of long complex genes, several of which play a role in DNA maintenance and repair. CDK12 is highly expressed in adult liver, kidney, testis, ovary, spinal cord and within specific adult brain regions with the highest expression being detected in cerebellum and caudate nucleus (1). CYCK-CDK12 and CYCK-CDK13 complexes show unique function with the CYCK-CDK12 playing a role in maintenance of genomic stability (2).
Gene Aliases:
CDK12: CDK12; CRKRS; CRK7; CRKR; KIAA0904
Genbank Number:
References:
1. Ko, T. K.et.al: CrkRS: a novel conserved Cdc2-related protein kinase that colocalises with SC35 speckles. J. Cell Sci. 114: 2591-2603, 2001.
2. Blazek, D.et.al: The cyclin K/Cdk12 complex maintains genomic stability via regulation of expression of DNA damage response genes. Genes Dev. 25: 2158-2172, 2011.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~135kDa
M Kanke et al., Cohesin acetylation and Wapl-Pds5 oppositely regulate translocation of cohesin along DNA. EMBO Journal November 2016 10.15252/embj.201695756
Lu Huasong et al., Compensatory induction of MYC expression by sustained CDK9 inhibition via a BRD4-dependent mechanism Elife June 2015 10.7554/eLife.06535
Bhandaria Deepali et al., Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration?proliferation dichotomy PNAS July 2015 10.1073/pnas.1514157112
et al., The mechanism of BUD13 m6A methylation mediated MBNL1-phosphorylation by CDK12 regulating the vasculogenic mimicry in glioblastoma cells Cell Death & Disease December 2022
Cancer, Cell Cycle, Ser/Thr Kinases
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