BRAF is a member of the RAF family that is activated by members of the Ras family upon growth factor-induced stimulation. Active Ras can induce heterodimerization of cRaf and BRAF and this may explain the observed cooperativity of cRaf and BRAF in cells responding to growth factor signals (1). Activating mutations in the BRAF gene are present in a large percentage of human malignant melanomas and in a proportion of colon cancers. The vast majority of these mutations result in a valine to glutamic acid change at residue 599 within the activation segment of BRAF (2).
BRAF (Ex9-18), BRAF1, RAFB1, B-raf, B-raf 1, MGC126806, MGC138284
1. Weber, C K. et al: Active Ras induces heterodimerization of cRaf and BRaf. Cancer Res. 2001 May 1;61(9):3595-8.
2. Mercer, K E. et al: Raf proteins and cancer: B-Raf is identified as a mutational target. Biochim Biophys Acta. 2003 Jun 5;1653(1):25-40.
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Wua Zeng et al., Design, synthesis and biological evaluation of indole derivatives as novel inhibitors targeting B-Raf kinase Chinese Chemical Letters February 2014 10.1016/j.bmcl.2012.05.053
Bhandaria Deepali et al., Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration?proliferation dichotomy PNAS July 2015 10.1073/pnas.1514157112
Angiogenesis, Apoptosis/Autophagy, Cancer, ERK/MAPK Pathway, JAK/STAT Pathway, Ser/Thr Kinases