Overview:

TEV Protease is a highly sequence specific cystine protease originating from the Tobacco Etch Virus (1). The optimum recognition site for this enzyme is the sequence ENLYFQ(G/S) and cleavage occurs between the Gln and Gly/Ser residues (2,3). It is frequently used in affinity purification of proteins to remove affinity tags from recombinant proteins.

Genbank Number:

M15239

References:

1. Phan J, Zdanov A, Evdokimov AG, Tropea JE, Peters HK 3rd, Kapust RB, Li M, Wlodawer A, Waugh DS. 2002. Structural basis for the substrate specificity of tobacco etch virus protease. J Biol Chem. 27;277(52):50564-72.
2. Kostallas G, Löfdahl PÅ, Samuelson P. 2011. Substrate profiling of tobacco etch virus protease using a novel fluorescence-assisted whole-cell assay. PLoS One. 6(1):e16136.
3. Carrington JC, Dougherty WG. 1988. A viral cleavage site cassette: identification of amino acid sequences required for tobacco etch virus polyprotein processing. Proc Natl Acad Sci USA. 85(10):3391-5.